SUMMARY:Antiplasmodium properties of cisplatin [cis-platinum (II) diammine dichloride], a neoplastic drug, have been assessed in in vivo and in vitro model systems of malarial parasite. A well-tolerated dose of 6 mg/kg body weight of the compound cured the mice infected with Plasmodium berghei and the amount of cisplatin required for in vitro inhibition (IC50) of a chloroquineresistant Plasmodium falciparum isolate was smaller than either chloroquine or quinine. The minimum inhibitory concentration (MIC) needed to prevent the in vitro multiplication of asexual blood parasites was 30 ng/ml. Late ring and trophozoite stages of the erythrocytic cycle were the most susceptible, whereas schizont and early ring stages were the least sensitive to the toxic effect of cisplatin. Multiple smaller doses were more effective in curing malaria in mice than a single large dose. In a few of the mice treated with a single intraperitoneal large dose of 6 mg/kg body weight, there was a delay in appearance of parasitemia but most of them recovered completely but slowly. This compound exerts its toxicity mainly by randomly damaging and cross-linking DNA strands as shown by Southern hybridization with a synthetic oligonucleotide probe, which is a repeat sequence in the falciparum genome. The report clearly demonstrates the antimalarial potentials of this compound and suggests a closer evaluation of this and other related compounds, specially in combination with antimalarial drugs to probe their synergistic properties.241
Childhood nephrotic syndrome (NS) is a chronic illness characterized by relapses and remissions. In many there will be disability, caused by the disease and from the treatment in the form of immunosuppression or end stage renal disease. Progression to renal failure will require anticipation for dialysis and/or renal transplant. A noninvasive biomarker such as urine protein profile that accurately predicts responsiveness to steroid therapy would be valuable in predicting the course of nephrotic syndrome. Our study tries to correlate between the selectivity of proteinuria and the outcome of relapses and thus prognosticate the course of the disease. Few similar studies have been done in India.Our study is a cross-sectional hospital based study, comprised of a population of 43 children with nephrotic syndrome relapse, between 1 and 14 years of age. Blood and urine samples were collected, to look for selective or nonselective proteinuria using the Agarose gel electrophoresis (Age) method. The treatment for relapse was started with steroid and, outcome was then related to the selectivity of proteinuria.Majority of our patients had non selective proteinuria (NSP) 60.5%, the rest had selective proteinuria (SP) 39.5%.There was a significant relation between nonselective proteinuria and- 1: Hypertension (p =0.049); 2: Low GFR p<0.001(p =0.0005); 3: Steroid dependent nephrotic syndrome. (p =0.0199).Non selective proteinuria was more in children, above the age of 6 years (p=0.001).The nonselective proteinuria group took more time for remission pointing to the lesser degree of steroid sensitivity.Non selective proteinuria can be used as a biomarker to predict the course in childhood nephrotic syndrome. The higher prevalence of non-selective proteinuria could be due to severe forms of nephrotic syndrome in our study. Selective proteinuria had a better outcome.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.