The molecular weight distributions of 13-glucans from congress worts of the barley genotypes Minerva (feed barley) and Triumph (malting barley) at 0-9 days of malting were studied by gel permeation chromatography. The amount ofl3-glucan was estimated using a glucose determination method and a specific fluorometric method based on an increase in the fluorescence intensity of Calcofluor when binding to high molecular weight (> 10,000) ~-glucan. The amount of extracted 13-glucan was slightly higher for Minerva than for Triumph barley at all stages of germination. The elution patterns ofl3-glucan in congress worts made from the two harley genotypes were similar, although the high molecular weight 13-glucan analysed in worts from Triumph was decomposed during malting at a faster rate than in those from Minerva. The extracted high molecular weight 13-glucans were found to have molecular weights in the range of 104-l 06. The differences in the contents of high molecular weight ~-glucans cannot explain all the variation in viscosities of the worts.
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