PURPOSEThe goal of this study was to compare the adhesion of Candida albicans to the surfaces of CAD/CAM and conventionally fabricated complete denture bases.MATERIALS AND METHODSTwenty discs of acrylic resin poly (methyl methacrylate) were fabricated with CAD/CAM and conventional procedures (heat-polymerized acrylic resin). The specimens were divided into two groups: 10 discs were fabricated using the CAD/CAM procedure (Wieland Digital Denture Ivoclar Vivadent), and 10 discs were fabricated using a conventional flasking and pressure-pack technique. Candida colonization was performed on all the specimens using four Candida albicans isolates. The difference in Candida albicans adhesion on the discs was evaluated. The number of adherent yeast cells was calculated by the colony-forming units (CFU) and by Fluorescence microscopy.RESULTSThere was a significant difference in the adhesion of Candida albicans to the complete denture bases created with CAD/CAM and the adhesion to those created with the conventional procedure. The CAD/CAM denture bases exhibited less adhesion of Candida albicans than did the denture bases created with the conventional procedure (P<.05).CONCLUSIONThe CAD/CAM procedure for fabricating complete dentures showed promising potential for reducing the adherence of Candida to the denture base surface. Clinical Implications. Complete dentures made with the CAD/CAM procedure might decrease the incidence of denture stomatitis compared with conventional dentures.

Purpose: To evaluate the color stability of 3D-printed interim restorations with different surface treatments while immersed in various staining solutions or beverages (artificial saliva, tea, coffee, and wine) for 6 months. Material and Methods: An acrylic tooth was prepared for an all-ceramic fullcoverage restoration. A laboratory scanner was used to digitize the prepared abutment tooth. A virtual all-ceramic full-coverage restoration was designed on the digitized abutment tooth using a laboratory CAD/CAM software. Eighty abutment teeth and interim restorations were 3D-printed with tooth-colored photopolymerizing resin. The restorations were randomly allocated into two different groups: Polish and Optiglaze. For the Polish group, interim restorations were finished and polished with aluminum oxide finishers/polishers. For the Optiglaze group, one layer of nanofilled, lightpolymerizing protective coating was then applied. The 80 printed interim crowns were divided into 4 different groups depending on where they would be immersed: Artificial saliva, coffee, wine, and tea. After the sample allocations, there were 8 experimental groups. Each group was allocated with 10 specimens. Color measurements were obtained using a digital spectrophotometer in conjunction with The CIE L*a*b* system before the immersion and 6 months after the immersion. Two-way analysis of variance (ANOVA) was used to test the effects of surface treatment, immersion liquid, and their interaction on E at 6-month post-immersion. Fisher's protected least significant differences (LSD) was used as post hoc test to compare groups differences (α = .05). Results: There were significant effects of surface treatment [F(1,72) = 13.39, p = 0.000], immersion liquid [F(3,72) = 74.18, p = 0.000], and their interaction [F(3,72) = 16.33, p = 0.000] on E at 6-month post-immersion at the α = .05 level. The polish group showed significantly higher E than the Optiglaze group when immersed in coffee (p < 0.001) and wine (p = 0.015). The Optiglaze group showed significantly higher E than the polish group that was immersed in artificial saliva (p < 0.001). The wine group showed higher E than all other immersion liquid groups for both polish and Optiglaze surface treatments (p < 0.001 for all comparisons). L* showed a reduction that was significantly higher than the 2.0 acceptability threshold in the wine-polish (p < 0.001) and wine-Optiglaze (p < 0.001) groups.E showed a color change that was significantly higher than the perceptibility and acceptability thresholds for all the groups under polish surface treatment (p < 0.001).E also showed a color change that was significantly higher than the perceptibility and acceptability thresholds for artificial saliva, tea, and wine under Optiglaze surface treatment (p = 0.004). Conclusions: 3D-printed interim restorations showed significant discoloration after 6-months immersions in the artificial saliva and common beverages (including tea, coffee, and wine). Among all the chromogenic beverages, red wine caused the most signific...

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