Aim:To prepare and characterize Nanoparticles of Antibacterial Agent for Drug Delivery. Method: Levofloxacin Hemihydrate laden Nanoparticles were prepared using the Ionic gelation method followed by the micro fluidization technique using 400 bar pressure for 5 cycles. The F1 Nanoparticles suspension obtained was subjected to spray drying at a 1400 aspirator rate. The selected F1 Nanoparticles having an entrapment efficiency of 63.46% were characterised by a particle size analyzer, Zeta Analyser, SEM, AFM, FTIR, XRD, EDS, and In-vitro Cytotoxicity. The present research work was carried out during the year 2019-2020. Results: The particle size of F1 was found to be 159.0 nm. The F1 Nanoparticles showed a PI of 0.255, exhibiting the Nanoparticles as homogeneously dispersed colloids. The zeta potential of F1 was found to be 48.7 mV. Predicting the stability of nanoparticles SEM and AFM elucidate the surface morphology of F1. FTIR fingerprints show 1793.80 cm1 of carbonyl C=O, 2881.65 cm1 of aromatic C-H and 3535.52 cm1 of the O-H group of the carboxyl group of Levofloxacin. EDS detected oxygen, fluorine, and nitrogen as the elements present in Levofloxacin Hemihydrate and XRD confirmed an amorphous material with a few crystalline phases as diposite. There were no signs of eukaryotic cell disruption, showing non-toxicity and no bio reaction by F1Nanoparticles at 0.01g Inhibitory Concentration (IC50%) when characterised by an In-Vitro Cytotoxicity Study. Conclusion: Levofloxacin Hemihydrate laden Nanoparticles were lucratively prepared using Ionic gelation technique followed by the Micro fluidization further successful Characterization of Nanoparticles directs its potential in drug delivery for Treatment of Bacterial Infections.
The complex of Levofloxacin and Chitosan lead to formation of water soluble complex. This complex of Levofloxacin was then formulated into floating beads calcium alginate with maximum entrapment of Levofloxacin found to be about 75 %(f10) and the entrapment was found to be significantly higher as compared to the other formulations ( f1 to f9). In-Vitro Release studies of the beads f10 was found to significantly improve the release of Levofloxacin as compared to other formulations. The mean particle size of f10 microspheres and surface morphology were determined by SEM Resulting in Porous and Rough Surface of microspheres. The drug release kinetics were studied as zero order, first order , Higuchi , Koresmeyer-Peppas equations , good linearity was found in Higuchi's Equation (R 2 = 0.9310) indicating the release of the drug from Microspheres is based on Fickian Diffusion .
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