The treatment of wastewater for reuse is a potential solution to meet ever increasing urban, industrial, agricultural, and environmental demands across the world, where clean water availability is scarce. There are several traditional wastewater treatment processes that offer varying degrees of effectiveness in addition to presenting environmental, economic, and social disadvantages. Development of promising and inexpensive technologies to provide the reusable water in needful amounts using wastewaters as a cheap source of key nutrients and organic matter is required. Wastewater treatment by biological methods is becoming more important in the light of recovering value-added plant nutrients, heavy metals, biosolids, and bioenergy resources. Different types of solid contaminants in effluents can be removed simultaneously by pure cultures or mixed microbial consortia. Based on the structural organization of microbial biomass, biological treatment systems are classified into two types: dispersed growth system and attached growth system. Biological treatment methods associated with fixed-film growth have been recognized as highly effective and more energy efficient than suspended growth systems. This review discusses the recent breakthroughs in advanced biological wastewater treatment using both the systems, and also focuses on key energetic resources recovery driven by biological technologies.

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1The resistance of bacterial biofilms to antibiotic treatment has been attributed to the emergence of 2 structurally heterogeneous microenvironments containing metabolically inactive cell populations. 3In this study, we use a three-dimensional individual-based cellular automata model to investigate

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The response of bacterial biofilms to treatment with antimicrobial agents is often characterized by the emergence of recalcitrant cellular microcolonies. We present an individual-based model to investigate the biophysical mechanisms of the selective resistance that arises within the biofilm and leads to a spatially heterogeneous response upon treatment with antibiotics. The response occurs in 3 distinct phases. In the first phase, the subpopulation of metabolically active cells diminishes due to antibiotic-induced cell death. Subsequently, in the second phase, increased nutrient availability allows dormant cells in the lower layers of the biofilm to transform into metabolically active cells. In the third phase, survival of the biofilm is governed by the interplay between 2 contrasting factors: (1) rate of antibiotic-induced cell death and (2) rate of transformation of dormant cells into active ones. Metabolically active cells at the distal edge of the biofilm sacrifice themselves to protect the dormant cells in the interior by (1) reducing local antibiotic concentrations and (2) increasing nutrient availability. In the presence of quorum sensing, biofilms exhibit increased tolerance compared with the quorum sensing-negative strains. Extracellular polymeric substance (EPS) forms a protective layer at the top of the biofilm, thereby limiting antibiotic penetration. The surviving cells, in turn, produce EPS resulting in a feedback-like mechanism of resistance. Whereas resistance in QS − biofilms occurs because of transformation of dormant cells into metabolically active cells, this transformation is less pronounced in QS + biofilms, and resistance is a consequence of the sequestration of the antibiotic by EPS.

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