The aim of this research work was to develop a simple, accurate, sensitive and validated Ultra Violet (UV) spectrophotometric assay using the multivariate regression method for the analysis of Cilnidipine. This multivariate calibration technique was based on equations constructed using linear regression analysis using the correlation between absorbance and concentration at five selected equidistant wavelengths. Cilnidipine had a maximum absorbance at 240 nm. The findings were statistically analyzed for significance. A linear plot in the concentration range of 3-9 μg/mL, with a regression coefficient of 0.999 was obtained. The % RSD for intra-day and Inter-day precision were 0.4558 and 0.6099, respectively. The assay was determined and found to be 99.1% - 101.67% % w/w. Keywords: Cilnidipine, Antihypertensive agent, UV spectrophotometry, Multivariate calibration, Assay, ICH guidelines).
Oxetacaine is a potent local anesthetics used to relieve pain associated with peptic ulcer. The current method details about a rapid, accurate and precise HPTLC technique for the assessment of Oxetacaine in Pharmaceutical formulation. Chromatographic resolution was carried out on precoated HPTLC plates (Silica gel 60 F254 on Aluminum plate) employing methanol: water: glacial acetic acid (8: 1.8: 0.2 v/v/v) as mobile phase. Densitometric assessment was carried out at 220nm [Camag TLC Scanner III with winCATs software (version – 1.3.4)]. The drug was identified with a Rf value of 0.61. The reliability of the projected method was ascertained by evaluating various validation parameters as per ICH guidelines. The proposed technique can evaluate ten or more formulation units concurrently in a single run and affords a more rapid and cost-effective QC tool for regular analysis of Oxetacaine in pharmaceutical formulations.
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