Objective: To investigate the wound-healing potency of the Lakshadigana Taila on the excisional wound healing process in experimental study of albino rats, in comparison to 5% Povidone Iodine solution and untreated group. Materials and Methods:Albino rats of either sex weighing between 200 and 300 gm were divided randomly into three groups (n=6). The animals were anesthetized with anesthetic agent Thiopentone Sodium 25 mg/kg intra peritoneal. The skin of albino rats was saved on one side of paravertebral region. The skin was disinfected using cotton and alcohol wipes. Excisional wound, full thickness skin wounds of diameter 10 mm were created in the paravertebral area. The following medications were applied topically to the wound once daily for a maximum of 16 days. In Group I (trial group) Lakshadigana Taila was applied on rats, in Group II (positive control), 5% Povidone Iodine solution was applied on rats and Group III (negative control) served as untreated. Wound was measured on days 0, 4, 8, 12 and 16 of the experiment and histopathological of H and E stain study done on day 4 and 8, 12 and 16. Statistical analysis was done using analysis of One Way ANOVA followed by Post Hoc Test and P <0.05 was considered significant and <0.001 highly significant.Result: Area of wound was measured in all three groups at different intervals and found that the rate of wound contraction was statistically highly significant in all groups (p <0.001). Rate of wound contraction was faster in Group I than in Group II and III.Conclusion: In the present study, topical application of Lakshadigan Taila accelerated healing of excisional wound in albino rats.
Man is the superior most creature of God, having a sufficient intellectual mind to discover new things and concurrently overcome the existing problems. So from very ancient time different methods of wound care have been described. Wound is a separation or break in continuity of skin, mucous membrane or tissue caused by physical, chemical or biological insult. Wound infections continue to represent a major medical problem, both in terms of how they affect the outcome of surgical procedures and for their impact on the length of hospital stay and medical costs. In Ayurveda, an infected wound may be co-related with dushta vrana. Sushruta has explained that "vrana" (wound) is so called as it covers the site and the scar and even after healing, does not disappear till the person lives. Proper wound care is necessary to prevent infection and to promote healing of the wound. In this paper, emphasis has been given to dushta vrana and its management description according to Samhita and an attempt has been made to explain the infected wound according to modern concept. Gond P et al.born from the doshas and agantu (Traumatic) -caused by sharp weapons, implements, stone, baton, nails, teeth, horns, poisons, aruskara, etc. Even this (agantu vrana) when gets vitiated by vata and other dosha becomes a nija vrana. Again, he described types of vrana are of two kinds: duhsta (vitiated) and suddha (non-vitiated); dushta means getting vitiated by the doshas.
In the present study, RTc, an aqua-alcoholic extract of Tinospora cordifolia, a potential radioprotector, was evaluated for its ability to protect peritoneal macrophages (PMs) against LPS-induced death in ex vivo conditions using DNA fragmentation and survival assays. The effect of RTc on nitric oxide (NO) and TNF- production by LPS induced PMs was also assessed. The interaction of RTc with DNA (plasmid relaxation assay) and its effect on sodium nitroprusside (SNP) generated nitric oxide (NO) were also analysed. LPS induced significant DNA fragmentation in a dose dependent manner, decreased macrophage survival (48.2 ± 2.2 % of control), and increased NO (32.4 ± 1.5 ?M nitrite/106 cells) and TNF- levels (1943.4 ± 94.7 pg/ml) significantly as compared to their respective control values. Pre-treatment with RTc (-1h) decreased DNA fragmentation (13.4 ± 0.4 %), increased survival (87.5 ± 4.2 % of control), and reduced NO (18.9 ± 0.7 ?M nitrite/106 cells) and TNF- levels (418.5 ± 19.5 pg/ml) significantly. RTc also inhibited radiation-induced relaxation of plasmid DNA and significantly quenched SNP-generated NO even at low concentrations (64.6 ± 3.6 % inhibition at 2.25 ?g/ml, p ? 0.001). Such diverse effects of RTc on PMs could explain the radioprotective efficacy of this herbal extract.
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