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In this present work, we described a bio-reduction method for the generation of silver nanoparticles (AgNPs) using aqueous leaf extract of Micrargeria wightii (M. wightii), which is a gifted alternative to other physicochemical routes. The prepared AgNPs were characterized by UV–visible spectroscopy (UV–vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray diffraction (X-RD), Transmission Electron Microscopy (TEM) with EDX and Dynamic light scattering (DLS). UV–visible spectrum showed a characteristic absorption peak at 440 nm of synthesized AgNPs. FT-IR analysis confirmed the existence of plant metabolites, which are responsible for the reduction of Ag (I) ions into Ag (0) NPs. X-RD pattern studies confirm the presence of the pure face-centered cubiccrystalline nature of Ag. Energy-dispersive X-ray (E-DX) spectrum showed the elemental composition of synthesized nanoparticles. Furthermore, TEM images confirm the formation of spherical shaped nano-silver particles with sizes ranging from 30 to 70 nm and supported by particle size analyzer, Dynamic Light Scattering (DLS). Thus, the present investigation provides an easy, eco-friendly and straightforward route for the synthesis of the antibacterial agent against Bacillus subtilis subtilis and Pseudomonas aeruginosa, with 15 and 13 mm zone of inhibition (ZOI) respectively.

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