Submicroscopic deletions in chromosome 19 have been rarely reported. We reported a male patient presenting with neurodevelopmental delay and facial dysmorphisms with a de novo 19p13.11p13.12 deletion of approximately 1.4 Mb. To date, there are seven cases with deletions overlapping the 19p13.11-p13.12 region described in the literature. A region of 800 kb for branchial arch defects in the proximal region of 19p13.12, and another minimal critical region of 305 kb for hypertrichosis, synophrys, and protruding front teeth have been proposed previously. We suggest that the shortest region of overlap could be refined to an approximately 53 kb region shared within all patients, encompassing part of BRD4 and AKAP8L genes and the AKAP8 gene. Based on the genotype-phenotype correlation of the present case and cases with overlapping deletions described in the literature, it was possible to recognize a consistent phenotype characterized by microcephaly, ear abnormalities, rounded face, synophrys, arched or upwardly angulated eyebrows, short nose, anteverted nares, prominent cheeks, teeth abnormalities, and developmental delay.
Background The chromosomal microarray analysis (CMA) is recommended as a first‐tier test for individuals with developmental delay (DD)/intellectual disability (ID) and/or multiple congenital anomalies. However, owing to high costs, this technique is not widely performed for diagnostic purposes in several countries. The aim of this study was to identify clinical features that could favour the hypothesis of genomic imbalances (GIs) in individuals with DD/ID. Methods The sample consisted of 63 individuals, and all of them underwent a detailed evaluation by a clinical geneticist and were investigated by the CMA. They were divided into two groups. Group A composed of 20 individuals with pathogenic copy number variants (CNVs); and group B composed of 43 individuals with normal CMA results or variants of uncertain clinical significance (VUS). Results Pathogenic GIs were found in 20 cases (32%), including 11 individuals with an abnormal karyotype, VUS was found in five individuals (8%) and the results were normal in 38 individuals (60%). Major anomalies were found in 15/20 (75%) individuals in group A against 35/43 (81%) in group B. Dysmorphisms (≥5) were found in 17/20 (85%) in group A and 41/43 (95%) in group B. The most frequent major anomalies detected in group A were congenital heart disease, epilepsy and renal malformation; and in group B, they were malformations of central nervous system, congenital heart disease, microcephaly, epilepsy and hearing impairment. There was no significant statistical difference among the frequencies in groups A and B. Conclusions Evidences point that every individual with DD/ID, with no specific clinical suspicion, should have screening for GIs as a first‐tier test, regardless of the presence or absence of additional major anomalies or dysmorphisms. Future studies with a similar design would be helpful, especially in countries where the access to new technologies is still limited.
Submicroscopic deletions in chromosome 19 have been rarely reported. The aim of this study was to describe the genotype-phenotype correlation of a 19p13.11-p13.12 deletion. A male patient was referred for genetic evaluation by presenting neurodevelopmental delay and facial dysmorphisms. He is the first child of non-consanguineous parents. Pregnancy and delivery were uneventful. Anthropometrical data at seven-year-old were weight 25,4 kg (p75), length 120 cm (p50) and head circumference 47,3 cm (< -2SD). It was also observed malar hypoplasia, low-set and dysmorphic ears, bilateral preauricular tags, synophrys, short nose with anteverted nares and flat nasal bridge, down-turned corners of the mouth, long philtrum, micrognathia, high palate and congenital clubfoot. Abdominal ultrasonography showed pyelocaliceal ectasia, transfontanellar ultrasound revealed mild enlargement of magna cisterna and a small cyst ate nucleous caudatus. GTG-banding was normal and chromosomal microarray analysis (CMA), using the CytoScan HD Array (Affymetrix®, Santa Clara, CA, USA) showed an interstitial
A ata de defesa com as respectivas assinaturas dos membros da banca examinadora encontra-se no processo de vida acadêmica do aluno. Data: 07/12/2018Dedico este trabalho aos meus pais, Jaci e Fátima e aos meus irmãos, Lais e Luís Felipe, por todo apoio e carinho em todos os momentos da minha vida. AgradecimentosAgradeço, primeiramente, à Deus, pelo dom da minha vida e por ter me colocado em muitos desafios dos quais achei que não conseguiria, mas que com confiança Nele consegui superálos.Aos meus pais por todo apoio nesse período, pois sei que não foi fácil. Aos meus irmãos por sempre me ouvirem e, por me ajudarem encarar os desafios com um outro olhar.Ao meu orientador, Prof. Dr. Társis Antonio Paiva Vieira, por mais uma oportunidade e pela paciência e, por tudo que me ensinou até hoje, não sei como expressar minha gratidão à você! Muito obrigada Társis, de coração! À minha coorientadora, Profa. Dra. Vera Lúcia Gil da Silva Lopes, pela oportunidade e por toda ajuda e contribuição para esse trabalho.À banca examinadora por toda contribuição para dissertação.À funcionária do Laboratório de Citogenética Humana, Ilária, por toda ajuda para a realização desse trabalho. Por compartilhar seu conhecimento e pela amizade durante todo esse período, obrigada de coração! As, também funcionárias do Laboratório de Citogenética Humana, Nilma e Ana Paula e as alunas do laboratório, Samira e Flávia, por todo apoio e companhia durante esse período. E aos estagiários e alunos de aprimoramento que passaram pelo laboratório durante esse período.Aos pacientes e aos pais por aceitarem participar e por tornaram esse trabalho possível. RESUMOEm 2010, The International Standards for Cytogenomic Array (ISCA) Consortium recomendou o uso de Chromosomal Microarray Analysis (CMA) como o primeiro teste diagnóstico em indivíduos com atraso no desenvolvimento neuropsicomotor (ADNPM) / deficiência intelectual (DI), transtornos do espectro autista (TEA) ou anomalias congênitas múltiplas (ACM). Apesar desta recomendação e da utilidade da CMA para a caracterização de novas condições clínicas decorrentes de desequilíbrios genômicos (DGs), essa ainda não tem aplicação em larga escala em diferentes países, em razão de seus custos e necessidade de recursos humanos especializados. Os objetivos deste trabalho foram: reanalisar os resultados da técnica de CMA em indivíduos com ADNPM / DI e (ou) anomalias congênitas (AC) a partir de parâmetros atuais de interpretação dos dados; comparar as características clínicas entre o grupo com DGs patogênicos e o grupo com resultado de CMA normal ou variantes de significado clínico incerto (VUS); caracterizar DGs em indivíduos com cariótipo desbalanceado e realizar correlação genótipo-fenótipo em um caso com um DG raro. Essa dissertação é composta por dois capítulos, no primeiro foram realizadas a reanálise dos dados e a comparação dos sinais clínicos de 63 indivíduos investigados por CMA. Para a comparação dos sinais clínicos, a casuística foi dividida em dois grupos: grupo A -20 indivíduos com ADNPM / DI com DGs patog...
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