High and reproducible antibody response to miligram quantities of 2,4dinitrophenyl (DNP)-conjugated bovine IgG was elicited in pigs with the aid of a novel adjuvant composed of aluminium hydroxide, paraffin oil and Sorbitan trioleate (Al-Span-Oil). Repeated injections of antigen produced antibody levels up to 8 mg per ml of serum. The antibody level reached the maximum around the 12th day after first antigen injection, but around the 7th day after subsequent antigen injection. Isolated anti-DNP antibody was found to be chemically heterogeneous. It bound cDNP-lysine with a mean association constant of about lo6 M-'.
Tolerance to dinitrophenylated IgG in pigs 313 ratory [ 191 and by a study of Kapp and Benacerraf [20] which showed double PFC presence, although in lower numbers (0.5 t o 2 % of total PFC), between the 3rd and 5th day after immunization of mice with two non-crossreacting erythrocytes.Intraveneous administration of soluble 2,4-dinitrophenyl( DNP)-conjugated bovine IgC t o pigs led t o induction of tolerance t o this antigen. A single dose of 100 mg soluble conjugate per animal resulted in complete tolerance which lasted at least 80 days. Lower doses led t o partial tolerance. Induction of tolerance in pigs was shown t o be a process taking several days to complete. Animals exhibiting tolerance when challenged by the homologous conjugate still synthesized significant amounts of antibody when challenged by the carrier protein o r by a conjugate of the 2,4-dinitrophenyl group with a different carrier
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