Salmonellosis is one of the major health problem affect pigeons and protection of pigeons against this disease is very important, for this purpose the present work was planned to prepare an inactivated Montanide ISA-206 oil adjuvant vaccine from a locally pigeon isolate of Salmonella Typhimurium (S. Typhimurium) and determined of the optimal dose, which protect pigeon against salmonellosis. Evaluation of such preparation following the quality control tests revealed that it was free from any foreign contaminants, safe and immunogenic. Pigeons were divided into six groups, three of them vaccinated subcutaneously with single dose (0.5ml) of different bacteria concentrations (10 8 ,10 9 and 10 10 CFU/ dose) and the others three received 2 doses with 3 weeks intervals with same route and concentrations. The vaccination/challenge assay with a virulent S. typhimurium organism using 5x10 7 CFU/dose revealed that protection rates of vaccinated birds were 70% ,75% and 75% for the first three groups (Ia, IIa, IIIa) respectively, while the other three groups gave 80%, 85% and 85% protection(Ib, IIb, IIIb) respectively.Control groups could not withstand the challenge and protection rate were 15% and 20% respectively. The seroevaluation showed that the humoral immune response developed against S. typhimurium in vaccinated pigeons was high. On conclusion the optimal dose of the prepared vaccine should be 10 9 CFU/dose applied twice with three weeks interval, which can cover the needed requirements and protect pigeon against salmonellosis.
A B S T R A C TThe objective of the present work is to prepare and evaluate the effectiveness of a locally prepared polyvalent vaccine against mastitis from the most common causes of mastitis. Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli were the most prevalent bacteria recovered from clinical and subclinical mastitis. Montanide ISA-206 adjuvanted inactivated polyvalent vaccine containing the three strains was prepared. Twenty pregnant cows were inoculated intramuscularly with the prepared polyvalent vaccine two months prior to calving and boostering at day 21 from the primary injection. Serum samples from vaccinated and non-vaccinated cows were collected at the 1 st , 3 rd , 8 th , 12 th , 16 th , 20 th and 24 th weeks post vaccination and evaluated immunologically using ELISA. The results showed that immune response was significantly higher in the vaccinated group than that of controls. These results could be indicated the safety and effectiveness of the vaccine in reduction of incidence and severity of clinical cases of mastitis but further studies should be done to elucidate the possibility of field application and effectively.
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