The aim of this study was to evaluate the capability of Lactobacillus reuteri NRRL 14171 and Lactobacillus casei Shirota to remove dietary acrylamide (AA) under simulated gastrointestinal conditions using a dynamic system. The effects of different AA levels or bacteria concentration on toxin removal by Lactobacillus strains were assessed. Thereafter, AA-removing capability of bacteria strains under either fasting or postprandial simulated gastrointestinal conditions was evaluated. Commercial potato chips were analyzed for their AA content, and then used as a food model. Average AA content (34,162μg/kg) in potato chips exceeded by ca. 34-fold the indicative values recommended by the EU. Toxin removal ability was dependent on AA content and bacterial cell concentration. A reduction on bacterial viability was observed in the food model and at the end of both digestive processes evaluated. However, bacteria survived in enough concentrations to remove part of the toxin (32-73%). Both bacterial strains were able to remove AA under different simulated gastrointestinal conditions, being L. casei Shirota the most effective (ca. 70% removal). These findings confirmed the risk of potato chips as dietary AA exposure for consumers, and that strains of the genus Lactobacillus could be employed to reduce the bioavailability of dietary AA.
The aims of this research were to study the applicability of flow cytometry to detect aflatoxin bound to Lactobacillus reuteri based on fluorescence intensity patterns, and to evaluate the aflatoxin/bacteria interaction by microscopy techniques based on fluorescent staining and disruption of the cell wall structure. Our results demonstrated a specific interaction between the AFB 1 bound to the cell surface and the FITC-labeled monoclonal antibody against the aflatoxin B 1 used. Microscopy assays confirmed for first time in a clear visual way the aflatoxin-bacteria interaction, and that aflatoxin binding causes changes that alter the bacterial cell surface. The methods applied in our study are suitable for detection of aflatoxin bound to the cell surface of Lactobacillus reuteri and possibly by other microorganisms, and proved to be a potential tool for research and clinical detection of human exposure to aflatoxins.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.