None of the patients with an excellent, indeterminate, or biochemical incomplete response to therapy prior to pregnancy developed structurally identifiable disease after a full-term delivery. Even though structural disease progression was seen in almost a third of the patients with known structural disease prior to pregnancy, only a minority of these patients had changes sufficient to warrant additional therapy. These data confirm that pre-pregnancy response to therapy status is an excellent predictor of pregnancy-associated disease progression in women previously treated for DTC.

BackgroundThe detection of insulin autoantibodies (IAA) aids in the prediction of autoimmune diabetes development. However, the long-standing, gold standard 125I-insulin radiobinding assay (RBA) has low reproducibility between laboratories, long sample processing times and requires the use of newly synthesized radiolabeled insulin for each set of assays. Therefore, a rapid, non-radioactive, and reproducible assay is highly desirable.MethodsWe have developed electrochemiluminescence (ECL)-based assays that fulfill these criteria in the measurement of IAA and anti-insulin antibodies (IA) in non-obese diabetic (NOD) mice and in type 1 diabetic individuals, respectively. Using the murine IAA ECL assay, we examined the correlation between IAA, histopathological insulitis, and blood glucose in a cohort of female NOD mice from 4 up to 36 weeks of age. We developed a human IA ECL assay that we compared to conventional RBA and validated using samples from 34 diabetic and 59 non-diabetic individuals in three independent laboratories.ResultsOur ECL assays were rapid and sensitive with a broad dynamic range and low background. In the NOD mouse model, IAA levels measured by ECL were positively correlated with insulitis severity, and the values measured at 8-10 weeks of age were predictive of diabetes onset. Using human serum and plasma samples, our IA ECL assay yielded reproducible and accurate results with an average sensitivity of 84% at 95% specificity with no statistically significant difference between laboratories.ConclusionsThese novel, non-radioactive ECL-based assays should facilitate reliable and fast detection of antibodies to insulin and its precursors sera and plasma in a standardized manner between laboratories in both research and clinical settings. Our next step is to evaluate the human IA assay in the detection of IAA in prediabetic subjects or those at risk of type 1 diabetes and to develop similar assays for other autoantibodies that together are predictive for the diagnosis of this common disorder, in order to improve prediction and facilitate future therapeutic trials.

Clinical studies consistently show that pregnancy is not associated with significant disease progression in newly diagnosed thyroid cancer, PMCs under active surveillance, or previously treated DTC.

The task of the work was geoelectrical studies using variations of the magnetotelluric (MT) field of the Kozloduy nuclear power plant (KNPP) region and the integration of its results with other geological and geophysical knowledge. This paper presents the determined interpretation parameters of the MT field. The KNPP is located on the right bank of the Danube River in close proximity to the river. This fact, together with the location of electrified railways determined the unique network of locations of observation points for MT field variations. Based on the analysis of Earthquake Catalogs of Bulgaria and international seismicity databases, a map of the seismicity of nuclear power plant areas was built. Over the past 50 years, about 750 earthquakes (mainly south of KNPP) have been recorded at a distance of 40—80 km from the KNPP. Two magnetotelluric stations GEOMAG-02 were used at measurement sites, but equipment for recording electrical channels was available only for one station (due to the lack of another set of non-polarizable electrodes). The MT field variations were observed at 21 points, which are located on the territory with sides approximately 30—35 km from east to west and 40—50 km from north to south. For all observation points on the profile, only the parameters of the vertical magnetic transfer function (VMPF) were determined, in the form of the real (Cu) and imaginary (Cv) parts of the induction vector. The steadily induction vector was defined for periods from 10—20 to 4900—10 800 s. For most points it was possible to estimate the values Cu, Cv with an error of 0.02—0.04 and AzCu, AzCv 3—5°. The analysis showed the presence of anomalous behavior of Cu, Cv in different intervals of periods at some points. In the shortest (about 20 s) and longest periods (600 to 1000 s), the Cu directions completely coincide and indicate the presence of anomalous conductivity of the quasi-longitudinal strike to the west of the study area. This behavior of the Cu vector is in good agreement with power isohypsum strike of the Cenozoic deposits. At intermediate periods of 50—200 s, the behavior of Cu is more complex. Approaching the zone of high seismicity, the direction of the Cu differs from the previous ones by almost 90°. On the Geoelectrical sections, obtained as a result of 1D inversions of MTS curves at 4 points located in the southern part of the region, anomalous layers are identified (ρ about 10 ohm · m, the depth of the center of the object is 15—20 km). It can be assumed that well-conducting objects in the Earth’s crust of the region, apparently, prevent the propagation of seismic waves from nearby earthquakes to the north towards the KNPP.

Undoubtedly, one of the most interesting problems of modern electrocardiography is the question of intra-atrial blockade.

Ischemic necrosis (Ziegler's myomalacia cordis) is, no Gieson, a consequence of occlusion of a branch of the coronary arteries of the heart. The cause of occlusion may be embolism or thrombosis of the vessel, calcification or fibrosis of its wall.

Polyuria and associated polydipsia are known to be the main, and often the only, symptoms of diabetes insipidus. The amount of urine excreted may reach enormous figures - 43 liters in the case of Trousseau. Specific gravity is at a very low level, reaching 1001 and below. Together with this disturbance of water exchange, the exchange of sodium chloride is usually disturbed, and these two processes, in fact, characterize diabetes insipidus. Regarding the origin, systematics, and pathogenesis of these basic symptoms there is a large literature, but even on the main questions there are still fundamental discrepancies.

Digestive leukocytosis and leukopenia are considered by everyone at the present time, as a result of distributional fluctuations in the content of leukocytes in the vascular bed.