Herpetomonas samuelpessoai is a non-pathogenic protozoan that shares important antigens with Trypanosoma cruzi (the agent of Chagas' disease) and which shows three developmental stages: promastigote, paramastigote and the highly differentiated form opisthomastigote. Due to the difficulties in obtaining the last form, its physiology and biochemistry are not well understood, and procedures which can induce the process of differentiation promastigote-opisthomastigote are desirable. In this work we show that illumination of H. samuelpessoai with white light in the presence of methylene blue and oxygen (photodynamic effect) triggers the process of differentiation in a very efficient manner (the cultures show up to 70 per cent of the cells in the opisthomastigote form). We also observed that illumination alone and incubation with methylene blue in the dark can trigger the process but in levels markedly lower than illumination in the presence of the dye.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.