Black gram (Vigna mungo (L.) Hepper) cv. Regur was grown to maturity in three potted soils [Badgingarra (Sl), Lancelin (S2) and Quailing (S3)] with (+B) or without (-B) added H3BO3. The subsequent germination and vigour of seeds from treated plants was tested by germinating them for 7 days with 2000 8M CaSO4 and 98M H3BO3and, for S2 and S3 only, by staining with tetrazolium. No symptoms of B deficiency were observed in plants on any soil:leaf B concentrations at early vegetative and flowering stages were above values regarded as critical for this species (7-8 mg kg-1). On S1, B depressed pod and seed dry matter (DM), weight per seed and % hard seed (14 v. 58); it enhanced % non-viable imbibed seed (18 v. 5) and abnormal seedlings (37 v. 2). On S2, -B had no effect on reproductive yield or % hard seed but increased the % abnormal seedlings (32 v. 0) and depressed the tetrazolium rating for seed vigour, and weight per seed. No effects of B were observed on seeds from plants grown on S3 soil. When considered over all replicates of all treatments, a B concentration of 6 mg kg-1 DM seed was critical for viability of imbibed seed and production of normal seedlings. The results suggest that the subsequent germination and morphology of seeds and seedlings are more sensitive to low B supply during seed production than are growth of the plant and the amount of seed produced. It is thus likely that black gram crops low in B but with no symptoms of B deficiency may produce seed with low % germination and a high % abnormality in their seedlings.
The effects of the herbicides Hoegrass and Glean on the uptake of zinc by wheat were examined in two glasshouse experiments. Application of the active ingredients chlorsulfuron and diclofop-methyl decreased shoot weight, root weight and zinc uptake similarly to the herbicides Glean and Hoegrass. It can therefore be concluded that the effects of Hoegrass and Glean on zinc uptake in wheat can be attributed to these active ingredients and not to other constituents of the commercial herbicide preparations. Effects of chlorsulfuron on zinc uptake in soil were different from those obtained in solution. In soil, chlorsulfuron decreased both zinc concentrations in the youngest emerged blade and zinc content of shoots, while in solution where uptake is not likely to be limited by the rate of zinc diffusion to sites of absorption, there were no significant effects on zinc uptake. However, in both soil and solution, shoot and root weights were decreased by herbicide, particularly at low rates of zinc supply. Roots were shorter and thicker when herbicide concentrations in solution were increased.
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