A simple, sensitive and specific UFLC method was developed to estimate Chlorhexidine in bulk drug. Acetonitrile and Water were used in 60:40 v/v ratio as mobile phase. The flow rate of eluent was fixed at 0.8 mL/min. Absorbance was monitored at λmax of 235 nm. A reverse phase column C18, (250mm x 4.6mm i.d., 5µm) was used as stationary phase. The retention time was found to be 2.99 minutes. The linearity range of Chlorhexidine was found to be 1-6 μg/ml at 235nm wavelength.