Virtual poster abstracts intervention will consist of low doses of hydroxychloroquine (Plaquenil) 200mg tablets every 24 hours for 15 days, with a follow-up of 21 days after inclusion in the study. Inclusion criteria will consist of pregnant women older than 18 years of any gestational, with positive SARS-CoV2 infection by PCR testing, and a normal electrocardiogram. The primary outcome as the proportion of severe pneumonia (defined by the American Thoracic Society Criteria) will be evaluated between the control group and the experimental group using relative risk (RR) and absolute risk increase (ARI) in the established period of time plus their respective 95% confidence intervals. (95% CI). Results: We hypothesize that pregnant women with non-severe COVID-19 taking hdroxychloroquine will have an 8% lower risk for progression to severe pneumonia compared to those taking placebo. Conclusions: This study will provide crucial information on the effectiveness of hydroxychloroquine for the prevention of severe COVID-19 pneumonia, and if proven effective, the information of this study will help save the lives of these pregnant women by preventing a severe form of the disease.
Objectives:The aim of this study was to describe the placental lesions associated to SARS-CoV-2 infection by means of synchrotron X-ray phase-contrast imaging (X-PCI). Methods: Two blocks of placental tissue containing each a full thickness of placental parenchyma fixed by 10% buffered formalin were obtained from a laboratory confirmed SARS-CoV-2 positive placenta from a case of severe maternal COVID-19 and stillbirth at 37 weeks of gestation, and a second from a SARS-CoV-2 negative normal pregnancy. The two samples were introduced in a tube with deionised degassed water (figure A) and imaged in 3D with X-PCI at TOMCAT beamline (SLS, Switzerland) at an energy of 20 keV using a multiscale propagation-based protocol. First the whole sample was imaged at low-resolution (5.8 um pixel size). Then an area covering the full thickness from maternal to fetal side was selected for each sample (figure A) and imaged at high-resolution (HR) (0.65 um pixel size). Results: SARS-Cov-2 positive placenta shows lesions characterised by intervillous space collapse (figure C) and perivillous fibrinoid deposition (figure D) as compared to control placenta (figure B). Conclusions: Synchrotron X-PCI seems to be a powerful technique for multiresolution structural investigation of the placenta ex-vivo that could provide further insights in the SARS-CoV-2 infection in pregnancy.
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