authors request that the following clarification be noted. The Methods and Materials on p. 6987, line 28, indicated that mycobacterial cultures were grown in Middlebrook 7H9 broth (Difco) supplemented with ADC (albumin-dextrose complex) and 0.05% Tween 80. Middlebrook broth as originally formulated (23) and subsequently used for mycobacteria (24) contains glycerol. It has been brought to the authors' attention that the label on the Middlebrook-Dubos medium currently supplied by Difco states "if desired [each liter may contain] 2 ml glycerol or 0.05% Tween" (italics added). For the purposes of genetic transformation of BCG, the authors find that inclusion of 0.2% glycerol, as in the standard formulation, is required.

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