Engineered nanoparticles such as graphenes, nanodiamonds, and carbon nanotubes correspond to different allotropes of carbon and are among the best candidates for applications in fast-growing nanotechnology. It is thus likely that they may get into the environment at each step of their life cycle: production, use, and disposal. The aquatic compartment concentrates pollutants and is expected to be especially impacted. The toxicity of a compound is conventionally evaluated using mass concentration as a quantitative measure of exposure. However, several studies have highlighted that such a metric is not the best descriptor at the nanoscale. Here we compare the inhibition of Xenopus laevis larvae growth after in vivo exposure to different carbon nanoparticles for 12 days using different dose metrics and clearly show that surface area is the most relevant descriptor of toxicity for different types of carbon allotropes.
The worldwide increase of graphene family materials raises the question of the potential consequences resulting from their release in the environment and future consequences on ecosystem health, especially in the aquatic environment in which they are likely to accumulate. Thus, there is a need to evaluate the biological and ecological risk but also to find innovative solutions leading to the production of safer materials. This work focuses on the evaluation of functional group-safety relationships regarding to graphene oxide (GO) in vivo genotoxic potential toward X. laevis tadpoles. For this purpose, thermal treatments in H2 atmosphere were applied to produce reduced graphene oxide (rGOs) with different surface group compositions. Analysis performed indicated that GO induced disturbances in erythrocyte cell cycle leading to accumulation of cells in G0/G1 phase. Significant genotoxicity due to oxidative stress was observed in larvae exposed to low GO concentration (0.1 mg.L−1). Reduction of GO at 200 °C and 1000 °C produced a material that was no longer genotoxic at low concentrations. X-ray photoelectron spectroscopy (XPS) analysis indicated that epoxide groups may constitute a good candidate to explain the genotoxic potential of the most oxidized form of the material. Thermal reduction of GO may constitute an appropriate “safer-by-design” strategy for the development of a safer material for environment.
Extensive development of new applications using graphene based materials such as graphene oxide (GO) increases its potential release and occurrence into aquatic environments, raising the question of its biological and ecological risks. As standardized single species based assays fail to highlight t(l)(icological pathways implying interactions between organisms, the use of micro/mesocosms appears as a good solution to fill the lack of environmental realism inherent to these tests. In this work, experiments were achieved using microcosm systems to expose a reconstituted food chain to GO at environmentally relevant concentrations (0.05 and 0.1 mgL 1 ). The trophic chain was composed of a consortium of algae and bacteria as primary producers, chironomid larvae as primary consumers and decomposers while larvae of the amphibian Pleurode/es wa/tii constituted the secondary consumers. Monitoring of multiple ecotoxicological and ecological endpoints allowed to observe changes in bacterial communities while no toxic effects were noticed in chironomids. However, chironomids feeding behaviour changed as a consequence of GO contamination, leading to an increase in leaf litter consumption. Genotoxic effects were noticed in Pleurode/es larvae. This study highlights the importance of using such experimental systems to better encompass the ecotoxic potential of GO through the determination of t(l)(icological routes and consequences on ecosystem's functioning.
Despite the fast-growing use and production of graphene-based nanomaterials (GBMs), data concerning their effects on freshwater benthic macroinvertebrates are scarce. This study aims to investigate the effects of graphene oxide (GO) on the midge Chironomus riparius. Mortality, growth inhibition, development delay and teratogenicity, assessed using mentum deformity analysis, were investigated after a 7-day static exposure of the first instar larvae under controlled conditions. The collected data indicated that the survival rate was not impacted by GO, whereas chronic toxicity following a dose-dependent response occurred. Larval growth was affected, leading to a significant reduction in larval length (from 4.4 to 10.1%) in individuals reaching the fourth instar at any of the tested concentrations (from 0.1 to 100 mg/L). However, exposure to GO is not associated with an increased occurrence of mouthpart deformities or seriousness in larvae. These results highlight the suitability of monitoring the larval development of C. riparius as a sensitive marker of GO toxicity. The potential ecological consequences of larval size decrease need to be considered for a complete characterization of the GO-related environmental risk.
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