The aim of this study was to examine and compare the influence of preconditioning, perconditioning and postconditioning with creatine phosphate (PCr) on functional recovery and production of prooxidants of isolated rat heart subjected to ex vivo I-R injury on Langendorff apparatus. Wistar albino rats (male, n=40) were divided into 4 groups: control, and groups in which PCr (0.5mmol/l, 5 minutes) was perfused before (Pre group), after (Post group) or during (Per group) ex vivo induced ischemia. PCr application was associated with the great benefits on preserving cardiac contractility (in Pre group 100.96% for +(dP/dt max), 97.61.% for -(dP/dt max), in Per group 96.72% for +(dP/dt max), 95.60.% for -(dP/dt max) and in Post group 143.84% for +(dP/dt max), 104.36% for -(dP/dt max) in relation to the stabilization). In addition, PCr application prevented the rise of pro-oxidative markers during I-R injury in all therapeutic modalities. The most intensive benefits in the current investigation were observed when PCr was applied during the period of ischemia because the lowest fluctuations in the parameters of cardiac function and oxidative stress were observed. Overall, the results of this study highlight PCr-induced cardioprotection with promising prospects for future clinical use.
The present study strives to assess the cardioprotective role of phosphocreatine as an agent for postconditioning and perconditioning of isolated rat heart. Rat hearts (n=30) were perfused with a Langendorff apparatus and randomly assigned to three groups subjected to 20 minutes of global ischemia and 30 minutes of reperfusion: control group (untreated rat hearts), postconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of reperfusion), and perconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of ischemia). During the experimental protocol, cardiodynamic parameters were evaluated, while oxidative stress parameters such as superoxide anion radical, hydrogen peroxide, nitrites and index of lipid peroxidation were determined in coronary venous effluent. Postconditioning and perconditioning with phosphocreatine improved contractile function, heart rate and coronary flow, while the examined oxidative stress parameters in coronary venous effluent were significantly reduced in groups of treated rat hearts. The results of this study indicate that phosphocreatine has the potential as a therapeutic agent for perconditioning and postconditioning the heart in ischemia reperfusion injury.
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