common with preparations of thyrotropin and a-intermedin, increased the oxygen consumption of isolated leporine adipose tissue incubated in vitro."5 Summary.-Two metabolically active substances designated peptides I and II have been purified from a crude extract of porcine anterior pituitary glands. Electrophoresis on starch gel served as a guide during fractionation, which was accomplished using an anion-exchange resin and Sephadex G-50. Peptide I of molecular weight near 5,000 was homogeneous upon electrophoresis; peptide II was larger, was not freed of a contaminant, and was less active in causing fat mobilization and lipemia.
It is tentatively concluded from ultraviolet and x-ray studies that the two tryptic activities are mediated by overlapping "enzymatic sites." Crucial to this conclusion were studies of the factors which can modify the measured inactivation rates. The data are interpreted in the light of postulated mechanisms of inactivation.
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