Our study shows good correlation and agreement between CHr and RET-He and between %HYPO and DF-Hypo XE in evaluating CKD patients needing iron support.

No abstract

Most fetal hematologic parameters show a significant relationship with gestational age: a linear increase is evident throughout gestation for several hematologic parameters. A few reports have described reference values for umbilical cord blood reticulocyte counts performed with automated hematology analyzers. Our aim was to use automated hematology analyzers (ADVIA 120; Siemens Healthcare Diagnostics) to establish reference intervals for reticulocyte parameters in cord blood from healthy newborns of 34 to 42 weeks of gestation. We also investigated whether differences in reticulocyte parameters exist between the sexes and between different weeks of gestation. We enrolled 98 healthy, appropriate for gestational age newborns. In term infants, the reticulocyte percentage, the absolute reticulocyte count, and the reticulocyte hemoglobin content decreased significantly as the gestational age increased, but the maturation subpopulations did not change significantly. We found no significant differences between the sexes. In conclusion, our results contribute to the establishment of reference intervals for cord blood from full-term newborns that are measured with an automated hematology analyzer.

The peripheral reticulocyte count is commonly used as an indicator of the erythropoietic activity of the bone marrow. Manual counting provides results with a high degree of inaccuracy and imprecision. Automation of counting is therefore needed. The increase in the number of methods available requires however that the results from the various methods agree with one another. The aim of our study was to evaluate the analytic performance of two automated hematology analyzers by a parallel study. We compared the analyzers between them and with manual counting. We enrolled in our study a total of 100 healthy subjects and an additional 80 patients affected by various hematological diseases. Difference between methods is statistically significant: the reference intervals of ADVIA2120 are higher than the Sysmex XE-2100. The correlation between methods and correlation with the microscopic method are excellent and statistically significant. In conclusion, we can affirm that total automation of reticulocyte counts represents a definite improvement over microscopic counts. This study confirms the diversity of the reference intervals still exists in the new automated hematology analyzers.