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The influence of iron oxide nanoparticles decorating the external surface of multiwall carbon nanotubes (MWCNTs) on the amperometric sensing of glucose in solution is investigated. Oxidized nanotubes are decorated with iron oxide nanoparticles and then glucose oxidase is chemically bound to the MWCNTs for glucose sensing within physiological levels. MWCNTs which are only oxidized (without decorating nanoparticles) are used as reference. The results of the electrochemical characterizations consistently show that the presence of iron oxide nanoparticles decorating the surface of MWCNTs enhance the amperometric response and the sensitivity to increments in glucose concentration, when compared to non-decorated MWCNTs. The biosensor containing iron oxide decorated nanoparticles showed an amperometric sensitivity of 4.75 µA/mMcm 2 and an average response time of 6.6 s.