No abstract

Membrane-damaging action of laser irradiation comparing with membranotoxic activity of model xenobiotics (Novocam and Acrylarnide) has been evaluated in our experiments using Rosette-Forming Ability test (RFA) on rat blood lymphocytes, thyrnocytes, splenocytes for the assessment of membrane-mediated and receptor-mediated immune cells interactions.Infra-red laser irradiation (80 and 1500 Hz, 0.89 inkM) in vivo induced 2-fold increase of lymphocytes capable to form specific rossettes with xenogenous erythrocytes. T-lymphocytes were greatly sensitive to the laser influence. Acute laser exposure (1 28 sec) induced changes similar to Novocam action (1/2 LD50). 5-fold increase of the laser exposure time (especially for low frequency regime) resulted in more prominent changes in intercellular communication which were found to be similar to the action of Acrylamide (1/2 LD50). B-lymphocytes and splenocytes have been assumed as target cells for the action of laser with the frequency of 1500 Hz. Course application of IR or He-Ne laser induced decrease of RFA for all immune cells tested, and for blood lymphocytes , respectively. Thus, laser-induced changes in immune cells interaction may be connected with reversible injury of cell surface membrane followed by the dysregulation of cellular communication.Based on experimental data, the optimal regime of JR laserotherapy (0.89 and 0.63 tm) was chosen for the treatment of 200 patients with chronic decomnpensated tonsyllitis. Efficiency of laser application was confinned by cytological analysis of lacunes, laserodopplerofluometria, vegetative nervous system evaluation etc. and was found to be depend on inembranotropic activity oflaser irradiation.