Background
Circular RNAs (circRNAs) are non-coding RNAs resulting from back splicing of pre-mRNA. circRNAs exhibit higher stability with multiple functional modes. Thus, this circRNAs characteristic make it a promising entity for the development of diagnostic tools and therapies for human disease. In the present study, we mainly aimed to evaluate two circRNAs CDR1as and has-circRNA_105039 as noninvasive biomarkers for childhood dilated cardiomyopathy and ventricular septal defects patients.
Methods
Fold change of CDR1as and has-circRNA_105039 was detected by qRT-PCR in 101 participants. The diagnostic accuracy of CDR1as was determined by receiver operating characteristic (ROC) analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEEG) pathway analyses were performed to predict CDR1as/miRNAs and CDR1as/proteins interaction networks related to congenital ventricular septal defects (VSD) and childhood dilated cardiomyopathy (DCM).
Results
CDR1as showed significant higher fold change (FC = 2.8) in DCM group than controls and VSD groups. Experimental evidence-based GO and KEGG pathways analysis showed that has-miR-7-5p and hsa-miR-619 targeted 3'UTR of three mRNAs involved in MAPK signaling pathway.
Conclusion
Upregulated CDR1as may influence the level of has-miR-7-5p and hsa-miR-619 in childhood DCM patients, and further relieve the inhibitory effect of these miRNAs on kinases of the MAPK signaling pathway.
Aims: Acute rheumatic fever (ARF) is common between 5-15 years, uncommon with different presentation 25 in children below 5 years. The aim of this study is to assess the frequency and characterize the pattern of 26 presentation of rheumatic fever (RF) in Egyptian children younger than 5 years. Study Design: Retrospective study. Place and Duration of the Study: Pediatric department, cardiology division, Cairo University Children's Hospital, 5 years follow up. Methodology: We retrospectively reviewed the pre-completed data of 766 patients following up in the rheumatic fever clinic. Those with incomplete medical records were excluded. We compared between children younger than 5 years and those who are 5 years or older as regards their demographic data, clinical presentations, laboratory findings and echocardiographic findings. Results: We enrolled 667 patients; 17 of them (2.5%) were younger than 5 years (mean age 3.82± SD 0.393 years). The group of patients younger than 5 years old; included 10 females (58.8%) and 7 males (41.2%). Positive family history was encountered in 6 patients (37.7%). The most common presentations of the younger age group of patients were arthritis in 12 patients (70.5%), followed by carditis in 5 patients (29.4%), chorea in 3 patients (17.6%), and skin manifestations in 2 patients in the form of erythema marginatum (11.7%). Subclinical carditis was more common in younger children than the Research Article
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