In order to determine the effect of light-emitting diodes (LEDs) on plant metabolism, the present study examined the primary and secondary metabolite profiles of Vigna unguiculata L. Walp. sprouts that were exposed to red, blue, white, or a combination of red and blue LEDs using high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). A total of 39 hydrophilic compounds were identified and quantitated using GC-TOF-MS, and six phenylpropanoids and six carotenoids were quantified using HPLC. The plants grown under blue LED light contained the highest level of total carotenoids (253.72 ± 17.27 μg/g) and phenylpropanoids (2600.51 ± 4.90 μg/g). Thus, the current study provides a new approach for enhancing the carotenoid and phenylpropanoid production of V. unguiculata.
Foot-and-mouth disease virus (FMDV) 2A constructs have been successfully used for the production of “Golden Rice”, a β-carotene producing rice strain. However, to allay public fears and opposition to plants carrying a mammalian pathogenic viral sequence, 2A-like synthetic sequences from
Thosea asigna
virus and Infectious myonecrosis virus were used to coordinate the coexpression of carotenoid biosynthetic genes. Here, up to four carotenogenic genes encoding PSY, CRTI, BCH and BKT were concatenated and produced β-carotene, zeaxanthin, and ketocarotenoids (astaxanthin and adonixanthin) in transgenic rice seeds displaying color variation due to the difference in carotenoid content and composition.
Today, urban maglev train has a number of advantages. It is eco-friendly and cost-effective system because it generates little vibration, noise and dust compared to other systems. In the aspects of vehicle characteristics of propulsion, braking and levitation, however, the energy efficiency of the Maglev train is relatively low compared to other vehicles. In this paper, we analyzed and compared the energy efficiency of the electric braking by applying changing slip frequency to the current vehicle when control braking. Measured data results that changing slip frequency has performance to improve energy efficiency.
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