We have investigated the effects of brief, non-specific deuteration of Drosophila melanogaster by including varying percentages of ²H (D) in the H₂O used in the food mix consumed during initial development. Up to 22.5% deuterium oxide (D₂O) in H₂O was administered, with the result that a low percentage of D₂O in the water increased mean life span, whereas the highest percentage used (22.5%) reduced life span. After the one-time treatment period, adult flies were maintained ad libitum with food of normal isotopic distribution. At low deuterium levels, where life span extension was observed, there was no observed change in fecundity. Dead flies were assayed for deuterium incorporation by complete hydrolysis in hot 12 N HCl solution followed by subsequent high-performance liquid chromatography/mass spectrometry (HPLC/MS). Isoleucine and leucine residues showed a small, linear dose-dependent incorporation of deuterium at non-exchangeable sites. Although high levels of D₂O itself are toxic for other reasons, higher levels of deuterium incorporation, which can be achieved without toxicity by strategies that avoid direct use of D₂O, are clearly worth exploring.
A 20-year-old right hand–dominant male military cadet presented to the direct-access physical therapy clinic complaining of pain and swelling of his right hand, which was injured while competing in a team handball match the day before. Due to suspicion of a third metacarpal fracture, fluoroscopy was performed on the cadet's hand in the physical therapy clinic, and an apparent oblique fracture was noted. Confirmatory radiographs were ordered and the cadet was referred for orthopaedic consultation. J Orthop Sports Phys Ther 2020;50(1):44. doi:10.2519/jospt.2020.9034
A 22-year-old male US Military Academy cadet fell while sidestepping across the 8-ft-high bar portion of the indoor obstacle course. The cadet, in immense pain, was unable to bear weight immediately after the fall. Following examination by physical therapists within the fitness center, emergency medical service personnel transported the cadet to the emergency department for definitive care. Radiographs demonstrated impaction fractures of the tali, while computed tomography imaging of his ankles demonstrated Hawkins type 2 fractures bilaterally, indicating talar neck displacement and dislocation of the subtalar joints. J Orthop Sports Phys Ther 2020;50(7):409. doi:10.2519/jospt.2020.9098
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