A method is described for determining manganese down to 2.5 x 10-3 pg by using NN-diethylaniline as a colour reagent in an oxidation reaction. The concentration of diethylaniline is a significant factor in the intensity of the colour produced. The manganese content of whole blood was determined after extracting excess of iron with di-isopropyl ether. The manganese content of dog blood was shown to be in the range of 22 to 2 5 p g of manganese per 100 ml.THE increasing use of manganese compounds in various industries, such as steel and mining, and in the manufacture of batteries, ceramics, dyes and organometdic compounds, has aroused considerable interest in the hazards associated with toxicity of the dust produced. To investigate the metabolic functions of the element in conditions of health and disease, it is desirable to use a highly sensitive and precise method for its determination.Methods for manganese determination based on oxidation of Mn2+ to permanganate1~2s3s4s5 are not very sensitive as the amount of biological material available for analysis is limited. The most sensitive method involves neutron-activation analy~is,~ p 7 a procedure that is not practicable in most analytical laboratories. Other physical methods for the determination of manganese, in which flame-photometric and spectrographic techniques are used,899 are comparatively less sensitive.The ability of manganese to act catalytically in the oxidation of some organic compounds and thus form more intense colours than that of permanganate alone has been explored by many wo~kers.~0,11912113,~4,15 Most of the procedures involved are sensitive but either laborious or non-specific.The present method is based on the observations of Szebelledy and BartfaP that the oxidation of certain phenol and arylamine derivatives (the most sensitive of which is NN-diethylaniline) is catalysed by traces of manganese(I1) ion. Kun,17 Fore and Morton1* and Bolton, Cavender and Stachlg have developed similar procedures, but it was thought desirable to study in detail the various factors involved so that the method could be applied with confidence to biological materials in which traces of the element are present. This study was carried out during experimental investigations on manganese toxicity and the authors observed that the concentration of diethylaniline is a significant factor in the intensity of colour produced. Previous workersls p18 have obtained different results for the sensitivity of this catalytic method. An attempt has been made in the present study to explain this anomaly. The sensitivity can be increased considerably beyond that obtained by Fore and Morton18 by the use of a high concentration of diethylaniline. As it was not possible to determine manganese in whole blood by the method, because of the interference by iron, it has been modified by extraction of the blood iron before the determination. The complete method finally adopted is as follows. REAGENTS-NN-Diethylaniline solution, 0.0027 M-NN-Diethylaniline (430 mg) was accurately weighed and diss...
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