-Tobacco mosaic virus has been covalently conjugated with 500 -600 molecules of different hormones. The particles react with antisera against the hormones and with hormone receptors in target organs, on target cells, and on membrane vesicles. The compounds were used for cooperative affinity labelling and for the isolation of receptor-bearing vesicles. The binding of a tobacco mosaic virus/angiotensin II conjugate to adrenal cortex membrane vesicles was studied by dynamic light scattering and transient electric birefringence. Despite the complexity of the polydisperse systems and the fact that the membrane vesicles exhibit a Kerr effect and depolarize the light, methods were developed that allowed the observation and theoretical treatment of the effects due to specific binding.
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