Dermatopontin (DPT) is an extracellular matrix (ECM) protein with diversified pharmaceutical applications. It plays important role in cell adhesion/migration, angiogenesis and ECM maintenance. The recombinant production of this protein will enable further exploration of its multifaceted functions. In this study, DPT protein has been expressed in Escherichia coli (E.coli) aiming at cost effective recombinant production. The E.coli GJ1158 expression system was transformed with constructed recombinant vector (pRSETA-DPT) and protein was expressed as inclusion bodies on induction with NaCl. The inclusion bodies were solubilised in urea and renaturation of protein was done by on-column refolding procedure in Nickel activated Sepharose column. The refolded Histidine-tagged DPT protein was purified and eluted from column using imidazole and its purity was confirmed by analytical techniques. The biological activity of the protein was confirmed by collagen fibril assay, wound healing assay and Chorioallantoic Membrane (CAM) angiogenesis assay on comparison with standard DPT. The purified DPT was found to enhance the collagen fibrillogenesis process and improved the migration of human endothelial cells. About 73% enhanced wound closure was observed in purified DPT treated endothelial cells as compared to control. The purified DPT also could induce neovascularisation in the CAM model. At this stage, scaling up the production process for DPT with appropriate purity and reproducibility will have a promising commercial edge.
Traditional voting system has been replaced by electronic voting systems in most places increasingly. It is efficient, but not efficient enough in terms of cost and capacity. High Performance Computing (HPC) in Cloud computing is a relatively new concept which has been replacing the
traditional systems. The HPC has been widely used over the recent years because of its efficiency, reliability, speed and cost. Whereas in the traditional super computing system a lot of cost is involved. The ability of integration of HPC with cloud provided enormous growth in the area of
parallel processing and computing. This system may advocate to push the case of promoting electronic voting system for higher traffic scenarios with lower cost requirements. This paper proposes an idea of implementing a fully formed e-voting system integrated with both HPC and Cloud Computing.
Virtualization is used to set of connections and configure various operating systems on a single host at the same time. Now-a-days many technologies prefer Open source platform for virtualized infrastructure because it provides an enormous space to the user to implement their own module.
In this paper, we used piece based virtual machine (KVM) is the main open source finish virtualization arrangement on x86 equipment and it underpins all major working framework including Linux and windows.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.