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Total dissolved solids (TDS) comprising inorganic salts and organic matters are pollutants of concern to aquatic systems and water for human use. This work aimed to investigate the use of revolving algal biofilm (RAB) reactors as a sustainable and environmental friendly method to remove TDS from industrial effluents and municipal wastewaters. The wastewaters contained chloride, sodium, potassium, calcium, magnesium, and sulfate as the major components. The RAB reactors fed with synthetic industrial effluent with high TDS level demonstrated the best algal growth, with the highest TDS removal efficiency (27%) and removal rate (2,783 mg/L-day and 19,530 mg/m 2 -day). A suspended algal culture system only removed 3% TDS from the same wastewater. The TDS removal by the RAB reactors was considered due to several mechanisms such as absorption by the algae cells, adsorption by extracellular polymeric substance of the biofilm, and/or precipitation. Collectively, this research shows that the RAB reactors can serve as an efficient system in wastewater remediation for TDS removal. © 2019 Water Environment Federation • Practitioner points• Total dissolved solids (TDS) in wastewater are pollutants of concern. • The RAB reactors can remove TDS from various types of wastewater. • The RAB reactors removed TDS by adsorbing ions elements such as Cl, Na, K, Ca, Mg, and S. • The algal biomass absorbs ions through extracellular polymeric substance.

Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalgae take up and store wastewater P as polyphosphate as they grow in a partially submerged revolving biofilm, which may then be harvested and dried for use as fertilizer in lieu of mined phosphate rock. In this work, we isolated and characterized a total of 101 microalgae strains from active RAB systems across the US Midwest, including 82 green algae, 9 diatoms, and 10 cyanobacteria. Strains were identified by microscopy and 16S/18S ribosomal DNA sequencing, cryopreserved, and screened for elevated P content (as polyphosphate). Seven isolated strains possessed at least 50% more polyphosphate by cell dry weight than a microalgae consortium from a RAB system, with the top strain accumulating nearly threefold more polyphosphate. These top P-hyperaccumulating strains include the green alga Chlamydomonas pulvinata TCF-48 g and the diatoms Eolimna minima TCF-3d and Craticula molestiformis TCF-8d, possessing 11.4, 12.7, and 14.0% polyphosphate by cell dry weight, respectively. As a preliminary test of strain application for recovering P, Chlamydomonas pulvinata TCF-48 g was reinoculated into a bench-scale RAB system containing Bold basal medium. The strain successfully recolonized the system and recovered twofold more P from the medium than a microalgae consortium from a RAB system treating municipal wastewater. These isolated P-hyperaccumulating microalgae may have broad applications in resource recovery from various waste streams, including improving P removal from wastewater.

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