The DNA increment method, designed for measuring the increment in the amount of DNA after inhibition of initiation of fresh rounds of replication initiation was employed to measure the rate of deoxyribonucleic acid (DNA) chain growth in Mycobacterium tuberculosis H37Rv growing in Youman and Karlson's medium at 37 degrees C with a generation time of 24 h and also in relatively fast growing species like Mycobacterium smegmatis and Escherichia coli. From the results obtained, the time required for a DNA replication fork to traverse the chromosome from origin to terminus (C period) was calculated. The chain elongation rates of DNA of the three organisms was determined from the C period and the known genome sizes assuming that all these genomes have a single replication origin and bidirectional replication fork. The rate for M. tuberculosis was 3,200 nucleotides per min about 11 times slower than that of M. smegmatis and about 13-18 times slower than that of E. coli.
Much work has been done on the isolation, purification, and characterization of the RNA-directed RNA polymerase (EC 2.7.7.48) of cucumber mosaic virus (CMV)-infected cucumbers. Uninfected plants were reported to have no such enzyme, but we recently detected low levels of the activity in cucumber. Since tobacco and cowpea contain such an enzyme that is variably increased in amount by various virus (as well as viroid) infections, we assumed that this would also be the case upon CMV infection of cucumber. However, further purification and characterization of the RNA-directed RNA polymerases from healthy and from infected cucumber suggests that these are different enzymes. The presumed CMV replicase was obtained pure and consists of a major polypeptide of Mr 100,000 and minor components of Mr 110,000 and about 10,000. The Km is 5 pM ([3H]GTP) when tobacco mosaic virus RNA is used as template.The role of plant RNA-directed RNA polymerases (EC 2.7.7.48) in the replication ofRNA viruses remains uncertain. The RNA of cowpea mosaic virus, a virus resembling picornaviruses (1), appears to become replicated without participation of the cowpea RNA-directed RNA polymerase, although the amount of the enzyme is greatly increased by that virus infection (2-4). Several studies of viral replication complexes-particulate aggregates containing several proteins, membranous structures, and RNA-have given conflicting results in terms of containing the host enzyme and/or one or several viral gene products (5)(6)(7). In barley infected with brome mosaic virus (BMV), evidence for a virus-specific component in viral RNA replication was reported (5, 8). However, in tobacco no evidence was found for any role of a protein encoded by the infecting tobacco mosaic virus (TMV) in viral RNA replication (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20).The tobacco enzyme, as well as those of other plants, have molecular weights of 130,000-140,000. In contrast, the presumed viral RNA replicases have approximate molecular weights of 180,000 plus possibly 130,000 (TMV and turnip yellow mosaic virus) or 110,000 and possibly 70,000 [BMV and cucumber mosaic virus (CMV)]. Yet the molecular weight of the largest gene product of tobacco necrosis virus is only about 65,000. The need for both host and viral components for viral RNA replication is quite likely. This has been observed to be the case with the RNA bacteriophages and picornaviruses. Our present concern has been with cucumber, a plant reported to lack RNA-directed RNA polymerase, and the replication of CMV in this plant. We have found small amounts ofRNA-directed RNA polymerase in cucumber, partially characterized it, and compared it with the enzyme, produced in greater amount and obtained in pure form, from CMV-infected cucumber. It appears that the presumed CMV replicase has a molecular weight of about 100,000 and 110,000, whereas that of the cucumber enzyme is about 140,000. The earlier report that the Mr 100,000 enzyme differs in peptide pattern from the viral gene product corresponding ...
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