<p>We report efficient synthesis and photophysical properties of a novel fluorescent pyridothiazole based construct by a facile methodology and its application as a sensor for rapid, selective as well as sensitive detection of amyloid fibres. The novel compound has exceptionally high quantum yield and sensitivity. The fluorescence of pyridothiazole construct is completely quenched (Turn-OFF) upon addition of cupric ions and the fluorescence is recovered on addition of amyloidogenic peptides like A<i>β</i><sub>42 </sub>(Turn-ON). The control experiment with native and heat treated BSA suggest that the new probe binds to beeta/<i>β</i> sheets present in amyloid. The new probe offers direct visualization of fluorescence ‘Turn-OFF’ ‘Turn-ON’ under long UV without the use of sophisticated instrumentation. Hence, a very simple, facile and cost-effective methodology for the rapid detection of amyloid is presented and it may in all possibility pave the way for the design of a better probe for sensing amyloids than conventional ThT.</p>
<p>Herein, we report<b> </b>a novel fluorescent probe for the sequential detection of Copper II (Cu<sup>2+</sup>) and lactic acid. The probe based on acyl-thiourea derivative, N-((6-methoxypyridin-2-yl)carbamothioyl)benzamide (<b>1</b>) was synthesized, and its application as dual sensor for Cu<sup>2+</sup> and lactic acid is reported. The dual detection by the probe is possible since <b>1 </b>formed complex with Cu<sup>2+ </sup>and yielded yellow solution which disappeared after the addition of lactic acid. The probe exhibits yellow fluorescence with Cu<sup>2+ </sup>and LOD of Cu<sup>2+</sup> with fluorescence is as low as 0.1ppm. The fluorescence is quenched after the addition of lactic acid and LOD of fluorescence quenching by lactate is as low as 1 ppm. Further, structural modification in the probe <b>1</b> suggest crucial role of both pyridine and acyl-thiourea moiety in the binding of Cu<sup>2+</sup>. Interestingly, <b>1</b> also assembles to fluorescent fibers which show tunable emission properties. These fibers are broken in the presence of Cu<sup>2+</sup> and regenerated in the presence of lactic acid. Hence, disaggregation at molecular level might play a crucial role in causing yellow fluorescence in the presence of Cu<sup>2+</sup>. To, the best of our knowledge, this is the first report wherein a dual sensor for Cu<sup>2+</sup> and lactate ion is synthesized and it may in all possibilities pave the way for diagnosis of Cu<sup>2+</sup> associated disorders like Wilson’s disease and in the detection of elevated lactate levels which are associated with wide range of pathologies like mitochondrial diseases, cerebral ischemia and cancer.<b></b></p>
<p></p><p>We report self-assembly and photophysical properties of a new pyridothiazole based aggregation-induced-emission enhancement (AIEE) luminogen 4-(5-methoxy-thiazolo[4,5-b]pyridin-2-yl)benzoic acid (<b>PTC1</b>) and its application for the sensitive detection and monitoring of amyloid fibrillation. The self-assembling properties of the new AIEE probe are extensively studied by AFM and it was noted that as aggregation increases there is enhancement of fluorescence. The fluorescence of <b>PTC1 </b>is quenched upon addition of cupric (Cu<sup>2+</sup>) ions while the fluorescence is regenerated in the presence of amyloid fibers. AFM studies reveal that <b>PTC1</b> self associate/aggregate to hairy micelle structures which gets disrupted on the addition of Cu<sup>2+ </sup>and again reassembles in the presence of amyloid fibers. Hence, the fluorescence quenching and regeneration may be attributed to disaggregation and AIE respectively. Further, a comparative analysis of the performance of<b> PTC1</b> is done with the conventional ThT which confirms it to be a more sensitive probe for the detection of amyloid both in the presence and absence of Cu<sup>2+</sup>. Of note, a very simple, facile and cost-effective methodology for the detection of amyloid fibres is presented, wherein fluorescence quenching/enhancement can be visualized under UV without the use of sophisticated instrumentation techniques. To the best of our knowledge and literature survey, this is first report wherein the self-assembling properties of AIEE probe is studied extensively via microscopy and the photophysical properties compared w.r.t to the morphological transformations. The AIEE probe has been designed using an unusual pyridothiazole scaffold unlike the commonly used archetypal AIE scaffolds based on tetraphenylethene (TPE) and hexaphenylsilole (HPS) and hence, the work also has implications in designing new generation AIEE dyes based on novel scaffold reported. </p><br><p></p>
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