Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of 'Faro' and 'Titicaca' cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of 'Faro' and 'Titicaca', while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of 'Titicaca' (genetic distnaces from 7.5% to 55.9%) and in a single plant of 'Faro'(genetic distance 61.2% as indicated by SCoT technique).
S u m m a r yThe evaluation of Chenopodium quinoa cultivation effects in Poland has been compared with European research results. It was found that the conditions in Europe are favorable to Chenopodium quinoa cultivation. Poland has the mean length of the vegetation period. The maximum value of this trait was found in Sweden. The conditions in Bydgoszcz (Poland) are very favorable to the cultivation for green matter and favorable as far as the seed yield is concerned. The most favorable seed yield was recorded in Greece.
S u m m a r yBased on the evaluation of selected varieties of Chenopodium quinoa, their requirements were identifi ed; it was shown which varieties demonstrated the best effects in what conditions. It was found that the best varieties for Europe's conditions were E-DK-4-PQCIP and RU-5-PQCIP. E-DK-4-PQCIP variety showed the best effects under the conditions of cluster II which included e.g. Bydgoszcz (Poland) and cluster IV which included Southern European countries (Greece and Italy), while the best effects found in RU-5-PQCIP variety were identifi ed in cluster I which included Sweden. E-DK-4-PQCIP variety in cluster II showed a very high yield of green matter and a mean seed yield, and in cluster IV -a very high seed yield and a high yield of green matter.
Chenopodium quinoa Willd. is a new plant in the conditions of Poland. At harvest, it has a low content of dry matter, which makes it difficult to ensile. For this reason, a study was undertaken on the effect of microbial and chemical additives on selected quality characteristics of quinoa silage. Traits determining the suitability of the green forage and the influence of silage additives were assessed. A microbial additive and a chemical additive were used. The microbial additive contained bacterial strains of Enterococcus faecium, Lactobacillus plantarum and Pediococcus acidilactici (concentration 1.25•10 11 CFU•g-1) and was applied in the amount of 1 g•t-1 of fresh material. The chemical additive contained formic acid, propionic acid and ammonium formate, and was added in the amount of 5 l•t-1 of fresh material. The quality of the quinoa silage depending on the additive used was evaluated. The fresh material of Chenopodium quinoa Willd. contained only 6.42% water-soluble carbohydrates (WSC) in dry matter (DM) and its fermentability coefficient was 29.2. Lactic acid was predominant in the silage, while the content of acetic acid was average. In the control silage (without additives), small amounts of butyric acid (0.04% DM) were noted, so its quality according to the Flieg-Zimmer scale was good. No butyric acid was found in the silage prepared with additives, and their quality was very good. The control silage contained more N-NH 3 than the silage prepared with additives (P≤0.01). This indicated that the preservatives (silage additives) limited the process of protein degradation in the quinoa silage.
The material presented refers to the estimation of growth and the flowering (Chenopodium quinoa Willd.) under Polish conditions. The species has been a South-American pseudocereal cultivated in the traditional form in the Andean region for over 5 thousand years. Its advantage, apart from low soil and climate requirements, is that it shows high nutritive value. The Chenopodium quinoa protein is especially valuable with its amino acid composition which is better balanced than that of wheat or maize. It shows a better share of egzogenic aminoacids. Field examinations were carried out in 1999-2001 at the Experiment Station of Cultivar Testing at Chrząstowo. The experiment involved two cultivars from two various growing regions: America and Europe. Analyses were made to include development stages, plant growth dynamics, inflorescences development dynamics, inflorescence habit and flowering. Differences were recorded in the growth and development models of the cultivars researched. The European cultivar had a short compact inflorescence with a short flowering period, reaching 120 cm. American cultivar plants were high (160 cm); they showed a slower continuous growth, loose big-in-size inflorescence, and a long period of flowering. The plants ended their growing season over the flowering stage or seed formation. The adequate growth, the course of flowering and, as a result, a stable yielding of the European cultivar make the group suitable for the cultivation under Polish conditions. This breeding group should also be the parent material for the cultivation of the Polish cultivar of Chenopodium quinoa
Ferulic acid (FA) is a naturally occurring phenolic antioxidant that is widely used in the food, pharmaceutical, and cosmetic industries due to its low toxicity. Its derivatives also find numerous industrial applications and may have even higher biological activity than ferulic acid. In this study, the effect of the addition of FA and its derivatives—including vanillic acid (VA), dihydroferulic acid (DHFA), and 4-vinylguaiacol (4-VG)—on the oxidative stability of cold-pressed flaxseed oil and the degradation of bioactive compounds during oxidation was investigated. The results showed that FA and its derivatives affected the oxidative stability of flaxseed oil, but their antioxidant activity depended on the concentration (25–200 mg/100 g oil) and temperature of treatment (60–110 °C). Based on Rancimat test results, flaxseed oil oxidative stability predicted at 20 °C increased linearly with ferulic acid concentration, while its derivatives effectively prolonged the induction time at lower concentrations (50–100 mg/100 g oil). The addition of phenolic antioxidants (80 mg/100 g) generally showed a protective effect against polyunsaturated fatty acids (DHFA and 4-VG), sterols (4-VG), tocols (DHFA), squalene, and carotenoids (FA). The exception was VA, which increased the degradation of most bioactive compounds. It is believed that adding properly composed mixtures of FA and its derivatives (DHFA and 4-VG) can extend the shelf life of flaxseed oil and provide nutritional benefits.
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