Multiple species of Phytophthora, Phytopythium, and Pythium cause detrimental effects to soybean and one or more species have been recovered from symptomatic seedlings or roots, suggesting that these occur as species complexes. Our objective was to assess the species diversity and abundance of Phytophthora, Phytopythium, and Pythium associated with soybean seedlings at two different incubation temperatures from soils collected from the same 81 ha farm, but from five fields with a different history of production practices. To determine the effect of incubation temperature on the species detected, soils were baited with soybean seedlings at 15 and 25oC. At each temperature isolates were directly recovered from seedling roots and the associated rhizosphere soil was assayed via amplicon sequencing with oomycete specific primers. Regardless of incubation temperature or field site, Py. sylvaticum, and Py. ultimum were isolated through the baiting procedure and these along with Py. acrogynum, Py. attrantheridium, and Py. heterothallicum were detected with amplicon sequencing. Some Pythium species were more abundant at 15o C while others were favored by 25o C. This study demonstrated that the species diversity of Phytophthora, Phytopythium, and Pythium, detected on seedling roots and rhizosphere of soybean can be influenced by incubation temperature. This may explain, in part the differences in the species composition reported from previous surveys and suggests that production practices influence the species complexes of Phytophthora, Phytopythium, and Pythium.
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