12Activity patterns play an important role in the fitness of animals. Energy conservation, 13 physiological adaptations, prey availability, competition, and predation caused by predators 14 and humans are all important parameters influencing when, and where, animals are active. 15 Over time, however, a change in such external factors can lead to a shift in optimal activity 16 patterns. In this paper, we use camera traps to study the daily activity patterns of Eurasian 17 beavers (Castor fiber) reintroduced into an atypical, predator-free landscape. We explore if 18 and how beavers have adjusted their activity patterns in the absence of predators, and whether 19 this varies with day length and moonshine. Our results reveal that beavers in our study area 20 have a mainly crepuscular and nocturnal activity pattern, similar to animals in more natural 21 landscapes with predators. Changes in day length had only a limited effect on the duration of 22 beavers' activity, but, contrary to our expectations, beaver activity increased during bright 23 moonlight. Activity patterns were also clearly bimodal during nights with bright moonlight, 24 but unimodal during dark nights. The shape of their activity pattern did change throughout the 35 The timing of activity is crucial for most animals. Temporal changes in activity (hereafter 36 activity patterns) are an important adaptation that evolved in response to the time structure of 37 the environment, which changes with a 24 h periodicity. Activity patterns allow an animal to 38 anticipate the right time for a given behaviour or activity (Roll et al., 2006) and are influenced 39 by a number of factors, including an animal's physiological adaptations, prey availability and 40 distribution, competition, and disturbances caused by predators and humans (Kitchen et al., 41 2000; Yerushalmi and Green, 2009). There is evidence that the evolutionary arms race 42 between predators and prey for temporal niche occupation may have led to considerable 43 adaptive plasticity in temporal niche usage among even the earliest mammals (Hut et al., 44 2012). 45 46Plasticity in activity patterns has also been observed in a range of modern mammals, often 47 because of changes in the intensity of predation or competition, and over varying time scales.
48Svalbard reindeer (Rangifer tarandus platyrhynchus), isolated from predators for more than 49 5000 years, have no distinct peaks in activity at sunrise and sunset. This is expected for 50 animals maximizing energy intake rates in predator-free environments (Loe et al., 2007).
51Changes in behaviour and activity patterns in response to external cues have also been 52 recorded within shorter time spans. A coyote (Canis latrans) population that had historically 53 been exposed to human persecution shifted to higher levels of diurnal activity less than eight 54 years after persecution ceased (Kitchen et al., 2000). On an even shorter time scale, Fenn and 55 MacDonald (1995) observed diurnal activity in normally nocturnal wild rats (Rattus 56 nor...