Biodiesel involves the mixture of fatty acyl methyl/ethyl esters, produced from transesterification neutral lipids and if the origin of the source is from oleaginous micro organisms, then it is termed as micro diesel. In the present work, aiming to exploit fungi for biodiesel production, 12 fungal isolates were screened for lipid content by Sudan Black B staining method. Among 12 isolates, lipid rich five species viz, Mortierella alpina , M.ramanianna, M.vinancea, M.hyalina and M.verticella have been taken for fatty acids analysis by spectrophotometry, which revealed that the amount of free fatty acids were ranged from highest in M.alpina 35 ?moles of Oleic acid , 25 ?moles of Palmitic acid and 14 ?moles of Myristic acids to lower as much as 21 ?moles of Oleic acid , 18 ?moles of Palmitic acid and 16 ?moles of Myristic acids respectively in M.ramanianna. DOI: http://dx.doi.org/10.3126/ijls.v6i1.5721
Lipids produced by microalgal biomass can be grouped into nonpolar lipids and polar lipids, which can be easily converted into biofuels. Microalgal samples were collected from three different ponds of Bangalore and cultured in the laboratory to find the effect of different pH, temperature and media on the production of biomass and lipids. Among these, pH-9, temperature -25°C and Beneck’s media was most suitable for production of biomass (35.80 g/L) and lipids from the isolated microalgae Chlorella sp. compare to Chladospora sp. (13.33 g/L). Chlorella sp. Showed 0.32 (OD) at pH-9, 0.43 (OD) at temperature-25°C and 2.94 (OD) in Beneck’s media. Our result revealed that nutrient supply along with measured variables affects the production of biomass and lipids in different microalgae.DOI: http://dx.doi.org/10.3126/ijls.v8i2.10227 International Journal of Life Sciences Vol.8(2): 2014; 13-17
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