Combination therapy of multiple drugs through a single system is exhibiting high therapeutic effects. We investigate nanocarrier mediated inhibitory effects of topotecan (TPT) and quercetin (QT) on triple negative breast cancer (TNBC) (MDA-MB-231) and multi drug resistant (MDR) type breast cancer cells (MCF-7) with respect to cellular uptake efficiency and therapeutic mechanisms as in vitro and in vivo. The synthesized mesoporous silica nanoparticle (MSN) pores used for loading TPT; the outer of the nanoparticles was decorated with poly (acrylic acid) (PAA)-Chitosan (CS) as anionic inner-cationic outer layer respectively and conjugated with QT. Subsequently, grafting of arginine-glycine-aspartic acid (cRGD) peptide on the surface of nanocarrier (CPMSN) thwarted the uptake by normal cells, but facilitated their uptake in cancer cells through integrin receptor mediated endocytosis and the dissociation of nanocarriers due to the ability to degrade of CS and PAA in acidic pH, which enhance the intracellular release of drugs. Subsequently, the released drugs induce remarkable molecular activation as well as structural changes in tumor cell endoplasmic reticulum, nucleus and mitochondria that can trigger cell death. The valuable CPMSNs may open up new avenues in developing targeted therapeutic strategies to treat cancer through serving as an effective drug delivery podium.

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ABSTRACT:The study was conducted to findout the physico-chemical and bacteriological characteristics of Noyyal River and ground water quality of Perur, India. The river water and ground water (bore well and open well) samples were collected in and around Perur and analyzed for various physico-chemical and bacteriological parameters. The study indicated that the mean value of studied parameters, except phosphate, biological oxygen demand (BOD), chemical oxygen demand (COD) of the river water and ground water samples were within the permissible limit. The parameters include total suspended solids (TSS-132.2 mgl -1 ), BOD (9.55 mgl -1

Schematic illustration of the possible mechanism of pH based drug delivery system of DOX conjugated PEGylated PdNPs induced apoptosis in HeLa cells.

Schematic representation of crocin extraction from saffron stigmas and possible mechanism of pH based crocin delivery system of PEG-SeNP induced apoptosis in lung cancer cell.