Introduction:
Angiotensin I converting enzyme (ACE) insertion and deletion (I/D) polymorphism has been implicated in the pathogenesis of osteoarthritis (OA). In recent years, numerous genetic factors have been identified and implicated in OA. In this Asian Indian population-based study, we aimed to evaluate the relationship between ACE (I28005D) gene polymorphism and primary OA. We performed a case-control association study to identify and explore the correlation between clinically, radiologically diagnosed individuals with primary knee OA and the ACE I/D polymorphism.
Methods:
Genomic DNA was isolated from 200 samples, including 100 OA cases and 100 healthy volunteers. DNA was amplified by polymerase chain reaction (PCR) using I and D allele-specific primers. PCR products were assessed via UV visualization of products electrophoresed on 2% agarose gels.
Results:
The groups differed significantly in genotype distributions (p < 0.05). The primary knee OA group showed a considerably higher incidence of the DD genotype and the D allele compared to the control group (OR = 2.14, 95% CI: 1.10–4.15, p = 0.02 and OR = 2.08, 95% CI: 1.39–3.10, p = 0.0003).
Conclusion:
The ACE gene polymorphism I28005D was found to be associated with primary knee OA in Asian Indian populations. This is the first study in India to report that the ACE gene polymorphism is a risk factor for early onset primary knee OA.
Introduction: Osteoarthritis (OA) is a multifactorial disease with genetic factors playing a crucial role, and it has been associated with a family history of obesity. G595C polymorphism in the sterol regulatory element-binding protein 2 (SREBP2) gene has demonstrated an association with knee osteoarthritis (KOA) patients. However, this polymorphism has been never explored in an Indian population. Hence, the current study aimed to examine whether G595C (rs2228314) polymorphism in SREBP2 gene was associated with KOA susceptibility in the South Indian Hyderabad population. Methods: G595C polymorphism was genotyped with 200 KOA cases and 200 healthy controls using polymerase chain reaction-restriction fragment length polymorphism analysis. Results: A significant association was observed between age, body mass index (BMI), and family histories in KOA cases and controls (p < 0.05). The current allele (C vs G; OR-2.8 [95%CI = 2.1-3.7]; p < 0.0001) and genotype analysis confirms the significant association with (GC + CC vs GG; OR-3.5 [95%CI = 2.3-5.3]; p < 0.0001 & GC vs GG + CC; OR-1.7 [95%CI = 1.0-2.9]; p = 0.02) KOA vs. control subjects. On stratification analysis, genotype CC and C allele were associated with KOA. Gender association failed to demonstrate positive genotype frequencies (p > 0.05). Multifactor-dimensionality reduction (MDR) analysis showed a positive association with BMI and G595C genotypes (p < 0.05); 51% of the homozygous variant CC genotypes were present in obesity subjects.
Conclusion:In conclusion, our findings suggest that G595C polymorphism in SREBP2 gene is associated with KOA in the South Indian Hyderabad population and presents scope for further investigation of the gene's function in KOA.
Recurrent anterior dislocation of shoulder is a common orthopedic problem. The pathology is well known i.e. bank arts lesion, hill sachs lesion and capsular laxity. In the literature more than 150 types of surgeries were proposed but with their own limitations and recurrence. In the modified Boytchev procedure an active biomechanically dynamic sling is created by rerouting the conjoint tendon of coracobrachialis and short head of biceps under the subscapularis and fixed with a screw. This creates a (1) buttressing effect over anterior portion of head of humerus and (2) improves the stretch proprioceptive reflex in the subscapularis muscle and thus early and effective contraction of subscapularis. MATERIALS AND METHODS: This paper discusses our experience and results in the treatment of recurrent anterior dislocation of shoulder in 22 patients (n=22) during the period Aug 2007 to Dec 2011, with age group of 21-49 years with mean age 30.6 yrs with a mean follow up of 96.3 weeks with mean preop dislocations 10.5.All patients were evaluated by Visual analogue score {VAS} and Modified American Shoulder and Elbow Surgeon Score {ASES} at each follow up. The scores were statistically compared at each follow up. RESULTS: There were no dislocations in any of the patients. All patients had significant improvement in visual analogue score and modified American Shoulder and Elbow surgeon score at the last follow up. There was no loosening of screw at all the follow ups. One patient had screw removal. There was no loss of shoulder range of motion. CONCLUSION: The technically simple modified Boytchev procedure is an effective surgical treatment for recurrent anterior dislocation of shoulder.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.