Background:In recent years studying of in vitro toxicity encompass their possess significance to proceed any compound to further pharmaceutical procedure. In this we taken peptide which is supposed to be bind with DNA to produce anti-cancer possessions so it requires studying for its genotoxic possessions. Aims & Objective:To study Hemolytic and DNA nicking of anticancer peptide. Materials and Methods:Simple agarose gel was used to electrophoresis to determine the DNA nicking property and hemolytic assay also performed to ensure the safety of the peptide in biological system. Results:It was found that peptide is being safe even at elevated concentration 40 ng which cannot be achieved in biological distribution and the hemolytic assay shows the IC50 value 25.24 µg/ml which is also an awfully higher concentration in in vivo terms. Conclusion:So it is concluded the peptide have safe use in biological system.
As the prevalence of cancer persists, the development of new and more advanced therapies remains a main concern in the health research. In this work the development and validation of HPLC method for the quantification of anticancer peptide were checked for analytical validation parameters were accuracy, precision (repeatability and reproducibility), specificity, sensitivity (limit of detection and limit of quantification) and linearity. The accuracy and precision of the method have been tested and the coefficients of variation have been found to be less than 2% according to ICH validation guideline. The linearity range was found to be 5-35 µg/mL, LOD-5.65 µg/mL and LOQ-17.1322 µg/mL respectively. In conclusion, a new RP-HPLC method has been developed and validated successfully for the quantitative analysis for the peptide in this study.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.