Abstract‘Algae Service for LIFE’, the project supported by the European Union, seeks to promote best practices in ecological service and the circular economy by implementing innovative complex system of three interlinked elements: i) prototypes for harvesting of cyanobacteria and macroalgae biomass; ii) distant methods for surveying of the blooms and defining hot-spots of algal agglomerations; and iii) restitution of harvesting costs by redesigning of waste algal biomass into valuable products. The current paper describes application of algal biomass part of the project by providing actions in redesigning of harvested waste biomass of cyanobacteria and macroalgae into potential valuable products for sustainable management and recycling of environmental resources. It also highlights the socio-economic aspects of the project and added value of the project for the European Union.
Disruption of Neisseria denitrificans cells by microfluidizer was optimized using a factorial experiments design. The pH, pretreatment time, cell concentration, NaCl, ethylenediamine tetraacetic acid (EDTA) and Triton X-100 concentrations showed significant impact on disruption process and the process was optimized using central composite design and response surface methodology (RSM). Investigation revealed optimum conditions: 90 min pretreatment at pH 9.0 containing 110 g L(-1) cells (dry cell weight), 50 mM NaCl, 10 mM EDTA, and 0.2% Triton X-100. At optimized conditions, the disruption rate increased twofold, up to 5.62 ± 0.27 × 10(-3) MPa(-a); meanwhile, yield of intracellular content was increased by 26%, with 1 g of cells resulting in 113.2 ± 8.2 mg proteins, 12.1 ± 0.7 mg nucleic acids, 21.0 ± 1.2 mg polysaccharides, 0.99 ± 0.08 kU glucose-6-phosphate dehydrogenase (G6PD), and 10,100 ± 110 kU restriction endonuclease NdeI endonuclease. Particle size distribution analysis revealed nearly twofold larger cell lysate particles with diameter of 120 nm. For optimal release of intracellular content, 9200 J/g of energy was needed (95% confidence), yielding 6900 J/g energy savings. Model equations generated from RSM on cell disruption of N. denitrificans were found adequate to determine significant factors and its interaction. The results showed that optimized combination of known pretreatment and disruption methods could considerably improve cell disruption efficiency.
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