Thirty-one potential miRNAs that belong to 16 miRNA families were discovered from more than 324 000 EST sequences of apple (Malus domestica). In addition, precise sequences, especially terminal nucleotides of the 16 apple miRNAs (mdo-miRNAs) in 16 families were validated by miR-RACE, a newly developed method for the determination of the potential miRNAs predicted computationally. The expression of these 16 microRNAs could be detected in apple young leaf, old leaf, young stem, flower bud, flower and developing fruits by quantitative RT-PCR (qRT-PCR) and some of them showed tissue-specific expression. Fifty-six potential targets were identified for the 16 apple miRNAs, most of which were transcription factors that play important roles in apple development. Twelve target genes were experimentally verified by qRT-PCR, with some exhibiting different expression trends from their corresponding microRNAs, indicating the cleavage mode of miRNAs on their target genes.

Abstract:Finger millet is one of the potential cereal crops that can contribute to the efforts of realization of food security in the Sub-Saharan Africa. However, scientific information available with regards to improving soil phosphorus supply and identification of P efficient varieties for the crops potential yield is limited. In order to investigate the effects of P levels on yield components and grain yield On-station field experiments were conducted in two sites of western Kenya during the long and short rain seasons of 2015. The experiment was laid out in a Randomized Complete Block Design in factorial arrangement with four levels of P (0, 12.5, 25 and 37.5 kg P 2 O 5 ha -1 and three finger millet varieties (U-15, P-224 and a local check-Ikhulule) and the treatments replicated three times. The increase of phosphorus levels significantly (P≤0.05) increased the grain yield over the control up to 25 kg P 2 O 5 ha -1 during the long rain seasons and 25 kg P 2 O 5 ha -1 during the short rain seasons in both sites. Interactions at P≤0.05 were revealed on the grain yield where improved variety P-224 at 25 kg P 2 O 5 ha -1 produced the highest grain yield of 4.74 t.ha -1 in Alupe and 4.77 t.ha -1 in Kakamega and the consistent results suggest that the combination is highly recommended. Therefore the use of judicious and proper rates of P fertilizers can markedly increase the grain yield of finger millet in western Kenya.

The aim of the study was to evaluate popular ALVs in two counties; i.e. Kiambu and Kirinyaga counties in Kenya. Focus group discussion (FGD) and a field survey were employed. The study was carried out in the two counties in Central Kenya between January and February; 2015. Purposive sampling method was used where 67 respondents (37 and 30) Kiambu and Kirinyaga counties respectively; were interviewed. One FGD was carried out in each county. The findings indicated that; about seven ALVs were cultivated and consumed in the two counties. Vegetable amaranth and nightshade were the most dominant indigenous vegetables in both counties (83.8% and 81.1%) in Kiambu and (93.3% and 83.3%) in Kirinyaga respectively. These were followed by, spider plants, pumpkin leaves, cow peas leaves and jute mallow at 54.1%, 35.1% 32.4% and 21.6%

Finger millet (Elusine coracana) accounts for 8% of the total area and 11% of the millet production worldwide.  It is grown on over 4 million ha globally, mainly for food purposes. Millions of people in the dry lands of Central and East Africa, and South of India depend on finger millet as an important source of food to them (CGIAR, 2001). Finger millet is one of the most neglected and underutilized crops. Additionally, the crop has received limited research attention compared to wheat, rice, and maize (FAO, 2011). Therefore, production challenges such as those caused by weeds like goose grass Elusine indica remain at large. Manual weeding is the commonly employed weed control method in finger millet production, but is expensive and labour intensive. The current study was set to evaluate the influence of weed management practices on finger millet growth and yield components. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replicates. The plots measured 2 by 2m with a border width of 1m. The treatments included Pendimethalin, Dimethyl amine, Metolachlor, Metribuzin, Atrazine (at three rates each 1.0, 1.5 and 2.0 l/ha), No weeding and Hand weeding. Data was collected on the number of weed species, weed biomass, number of basal tillers, herbicide phytotoxicity, weed and crop heights, number of panicles, weight of panicles, weight of un-threshed and threshed grains and 1000 grain weight. Data was analyzed using one-way ANOVA using GenStat version 15.1. Application of Pendimethalin at 1.5 and 2.0 L/ha Active Ingredient (AI), resulted in weed optimal control and least phytotoxicity. Results also indicated that the height of finger millet was significantly (p<0.001) higher where the herbicides were applied. Lower weed biomass was also positively correlated with higher crop height, more panicles, high unthreshed and threshed weights and a 1000 grain weight. Application of 2,4D at rates of 1.5L and 2.0L resulted in significantly taller plants 33.00 cm, than the other weed management methods. Finger millet under Pendimethalin 1.5 L gave the highest number of 86 panicles while Atrazine 2.0L and Pendimethalin1.5L methods of weed control, had significantly higher weight compared to all the other treatments. The 1000 seed mass across the treatments averaged 2.31 g while the on the untreated treatments had an average of 1.54 g. Weed control using pre emergence herbicides significantly (p<0.001) increased the yields of finger millet.