The current study has been designed to synthesis silver nanoparticles (SNPs) and Copper nanoparticles (CuNPs) using leaf aqueous extracts of Cadaba fruticosa an endangered medicinal plant as bioreducing agents. This method authorized the synthesis of SNPs, which was authenticated by Ultraviolet (UV)-visible Spectrophotometry, Fourier-transform infrared analysis, X-ray Diffraction (XRD) studies, Energy Dispersive X-ray, and Scanning Electron Microscopic (SEM) analysis. UV-visible spectra and visual observation exhibited the appearance of greenish brown and dark brown color indicates the synthesis of SNP and CuNP, after the treatment of Ag precursors. Further the, XRD report clearly shows that the synthesized SNPs were crystalline in nature. In addition, SEM analysis confirmed that AgNO3 solution produced SNPs and CuO2 solution produced CuNPs and their size was about 120–168 nm and 301–538 nm in SNPs and CuNPs, respectively. The leaf SNPs and CuNPs of C. fruticosa leaf aqueous extracts considerable antioxidant activities. The results antimicrobial activity exhibited that the bacterial and fungal growth was inhibited by both the samples in a dose-dependent manner.
Introduction: Plants produce various chemicals to protect themselves; but recent studies proved that many phytochemicals can also protect humans against infectious diseases. The present study was conducted to identify and characterize the phytoconstituents and gas chromatography–mass spectroscopy (GCMS) analysis of petroleum ether, chloroform, ethyl acetate, ethanol, methanol, and aqueous extract of leaves of Tephrosia villosa (L.) Pers. of family Fabaceae. Materials and Method: The leaf extracts were subjected to qualitative screening for primary and secondary metabolites as per standard methods; further, GC–MS analysis was carried out for the identification of secondary metabolites. Result: Preliminary phytochemical screening of leaf extract of T. villosa showed positive result for alkaloids, flavonoids and triterpenoids, saponins, glycosides, gum and mucilages, and fixed oils in methanolic extract which is followed by aqueous extract. In quantitative analysis, the highest amount of phenolic content was obtained in aqueous extract, tannin in petroleum ether, and flavonoids and saponins in ethyl acetate. GC–MS analysis of leaf extract revealed the presence of 53 bioactive phytochemical compounds. Conclusion: It can be concluded that the species contain effective phytochemical compounds, need further research on toxicological aspects to develop a safe drug.
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