At the end of July beginning August 2014 an emergent bluetongue virus serotype 4 (BTV-4) was introduced in Albania and bluetongue disease (BTD) outbreak affected sheep, goats and cattle populations. Materials and methods:In this study, there were include data collected from 210 cattle, 56 of them shown clinical sign and were investigate closely. This subpopulation was part of cattle that randomly were tested for presence of total IgG by employing a competitive ELISA (cELISA) method. ELISA positive samples were tested by serum neutralization (SN) assay for nine different bluetongue serotypes.Results: Out of 210 individual bovine sera samples, 128 sera blood samples were positive in cELISA test for presence of total IgG against BTV. The most prevalent clinical signs in animal that developed BT disease were reduction of milk production, fever, lameness, conjunctivitis and appetite lost. The case fatality resulted 5.4%. The SN test confirmed the c-ELISA results revealing the presence of antibodies against BTV serotype 4 in 62.5% (80 samples) of cELISA positive samples and the titer varies from 1:10 to 1:640. Conclusion:The epizootic outbreak of BT disease affected cattle apart sheep and goats and severe clinical disease was described. Majority of the clinically affected cattle were positive in cELISA and from nine serotypes known to be spread in Europe, only BTV serotype 4 was determine by serum neutralization test.
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