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Feed composition is one of the most influential factors affecting fatty acid profile of milk products. The aim of this study was to investigate the influence of linseed oil and linseed extrudate supplementation on fatty acid composition of goat prebiotic and probiotic yogurt drinks. Thirty six White Shorthaired dairy goats at the beginning of their third lactation period were divided into two experimental and one control group, each comprising twelve animals. Goats in the experimental groups were given either 55 mL/day of linseed oil or 120 g/day of linseed extrudate over a three week period. The results suggest that feed supplementation with linseed oil and linseed extrudate caused considerable changes in fatty acid profile of goat yoghurt drinks. The most important nutritional change which was observed was increased n-3 fatty acid content (P<0.001) and decreased saturated fatty acid content (P<0.001). α-linolenic acid was significantly elevated (P<0.001) in both groups (in particular in goats which feed was supplemented with linseed oil).

SummaryThis study evaluated the effect of the linearly described shape traits of goat udders on somatic cell count. In a herd of 487 white shorthaired goats, seven traits (udder symmetry, udder depth, udder width, teat length, teat placement, rear udder attachment and udder cleft) were assessed in relation to somatic cell count in milk. The average somatic cell count was 1.3 mill cells/ml when considering the environmental effects (month and year of performance testing, lactation number. The somatic cell count is influenced by the depth (p = 0.0015) and width (p = 0.0268) of the udder. The results demonstrate that some traits of the udder shape influence the somatic cell count and can be considered as functional traits indicating animal health and herd profitability. After further studies, the methodology for linear description of the udder could be used for other dairy goat breeds, not only in the Czech Republic.

Matrix-assisted laser desorption/ionization--post-source decay (MALDI-PSD) fragment ion analysis is frequently used for peptide sequence determination. PSD fragmentation is often changed or improved in terms of, e.g., sequence coverage, after derivatization. In this work, the influence of modification by an osmium tetroxide-bipyridine reagent (Os,bipy) on the MALDI-PSD behaviour of peptides is studied. The reagent modifies peptides specifically at tryptophan residues and oxidizes methionine to methionine sulfone and cysteine to cysteic acid. As a result the masses of some of the fragments are specifically shifted in case of peptides containing a methionine by +32 Da and, in cases of peptides containing a cysteine residue, by +48 Da. In addition, due to the change in protonation properties of a peptide after oxidation, fragments containing cysteic acid are in most cases totally suppressed. This effect significantly facilitates peptide sequence determination. Improvement of MALDI-TOFMS and PSD analysis after the reaction with Os,bipy is demonstrated for examples involving derivatives of humanin, a novel neuroprotective peptide.