Electrochemically activated glassy carbon electrodes were applied to the preconcentration and electroanalysis of Vitamin B 2 (VB 2 ) in multivitamin preparation. Voltammetric peak currents resulting from VB 2 species adsorbed on activated electrodes showed linear response for VB 2 concentrations in the range between 0.1 and 3.0 mM with a large slope of 12.2 A M 71 and a correlation coef®cient of 0.996. The reproducibility of the voltammetric measurements was usually less than 7 % RSD for ®ve replicate measurements. Common vitamin and metal species did not interfere in the determination.