SUMMARYUsing the patch-clamp recording technique, we observed that endothelin-1 (ET-1; 0.8-16nM) enhanced a voltage-activated outward current ('out) and induced periodic oscillations of inward current in smooth muscle cells isolated from small pulmonary arteries (200-400 ,um in diameter). Anion substitution experiments revealed that the ET-1-induced inward current was carried by Clions. Application of bosentan (10 #m; an ETA and ETB receptor antagonist) and FR 139317(1-10 uM; a selective ETA receptor antagonist) prevented initiation of inward currents or enhancement of Iout by ET-1. The ETB receptor agonist tetra-Ala-endothelin-1 (1 -20 nM) failed to evoke these responses. Caffeine (10 mM) induced a single transient inward current and prevented any further activation of inward current, or enhancement of Iout, by subsequent application of 16 nm ET-1, suggesting that these currents were mediated by Ca2' release from internal stores. Rapid intracellular release of Ca2' by photolysis of nitr-5 activated an inward Clcurrent and increased the magnitude of Iout These results demonstrate the existence of Ca2+_ activated Cl-and K+ channels in pulmonary arterial smooth muscle. The physiological role of these channels is at present uncertain, although their activation may be involved in the contractile responses of pulmonary arterial smooth muscle to ET-1.
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