Cold-pressed juices are claimed to contain higher levels of antioxidants and bioactive compounds compared to normally centrifuged ones. Herein, we evaluated the antioxidant capacity and the bioactive compound contents of some freshly prepared fruit juices, extracted by a cold-pressed juicer and compared them to those prepared by a normal centrifugal juicer. We observed no significant differences between cold-pressed and normal centrifugal juices in terms of the contents of bioactive compounds (ascorbic acid, total phenolic, and total carotenoid) and antioxidant capacity (ferric ion reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity). Storage at room temperature (∼28 °C) adversely affected the ascorbic acid, total phenolics, total carotenoids, FRAP and DPPH values of the cold-pressed juices within 48 h. However, under simulated home-refrigerated storage conditions, the antioxidant capacity, contents of bioactive compounds and physicochemical properties of the cold-pressed juices remained unchanged till day 5 post-storage. However, at day 6, most of the parameters exhibited a decreasing trend and reached their lowest values at day 7. Principal component analysis confirmed significant changes in the quality of juices at day 7 of storage related to the first two principal components (ascorbic acid and FRAP). Our results strongly question the claim regarding the superior quality of cold-pressed juices. Moreover, our findings provided compelling evidence regarding the possible adverse effects of long storage under home-refrigerated conditions on the quality of cold-pressed juices.
Caffeic acid phenethyl ester (CAPE) nanoparticles (NPs) with an average size of ∼40 nm obtained from TEM and binomial average sizes of ∼90 and ∼400 nm obtained from DLS were successfully produced by rapid expansion of subcritical solutions into liquid solvents (RESOLV). The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of CAPE and CAPE-NPs were determined by plate count method against 12 pathogenic and spoilage bacteria and 3 strains of yeast. Total phenolic content (TPC) and antioxidant activities of CAPE-NPs were quantified and subsequently investigated using two assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP). CAPE-NP-incorporated cellulose-based films were prepared and characterized. MICs and MBCs of CAPE-NPs against most bacteria and Candida albicans were 700 and 1400 μg/mL, respectively. CAPE-NPs yielded a TPC value of 426.74 μgGAE/mg and lower antioxidant activities than those of CAPE in ethanol (CAPE-EtOH), whereas BHT yielded lower FRAP than that of CAPE-NPs. The impregnation of CAPE into cellulose-based films was confirmed by FTIR spectra. Moreover, incorporation of only 0.5 wt % CAPE-NPs into the films resulted in an inhibitory effect against microorganisms. Fortunately, incorporation of higher concentration of CAPE-NPs-MC films led to a significantly higher antioxidant activity and vice versa. This indicated that CAPE-NPs significantly enhanced the antimicrobial and antioxidant activities of CAPE. The results show that the environmentally benign supercritical CO2 technique should be generally applicable to NP fabrication of other important bioactive ingredients, especially in liquid form. In addition, it is suggested that CAPE-NPs can be used to reduce the dosage of CAPE and improve their bioavailability and thus merit further investigation for bioactive packaging film and coating applications.
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