Vigna radiata β-amylase was immobilized onto activated woven Bombyx mori silk fabric with glutaraldehyde and amino groups enrichment made by chlorination and by treatment with sodium nitrate (NaNO 3). The immobilization of enzyme onto sodium nitrate treated and chlorinated woven Bombyx mori silk fabric was excellent by having 90% of retention of enzyme activity after the immobilization. The optimum conditions of immobilized enzyme were studied such as time of incubation, pH, temperature, substrate concentration and CaCl 2 concentration. Thermal stability of the enzyme was improved after immobilization which was 72°C as compared to free enzyme which was only 40°C. In addition, the immobilized enzyme has good storage stability and reusability by maintaining 60% of its activity up to 3-4 months.
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