Stingless bees are native to tropical region and produce honey which are high in moisture content. Compared to honey from honeybees, there are limited studies on honey derived from stingless bees. Hence, the aim of this study was to evaluate the chemical composition and antioxidant activities of stingless bee honey. Fifteen types of honey were collected from six states in West Coast of Malaysia and pollen analyses were carried out. Four types of unifloral honey samples produced by stingless bees were selected to determine their physicochemical and antioxidant activities including total phenolic, total flavonoid and ascorbic acid contents. Melissopalynological study of 15 honey samples collected from different states showed presence of both unifloral and multifloral origins. Honey samples collected from Apis mellifera (honeybee) combs had lower number of total pollen compared to samples collected from Heterotrigona itama and Geniotrigona thoracica (stingless bees). Jambul Merak honey contains the highest phenolic and flavonoid contents with greatest color intensity and has the highest antioxidant potential. This study highlights the chemical composition and biological activity of honey from stingless bees which may increase its commercial value or to be utilised as potential functional food ingredient.
Rice leaffolder (RLF) (Cnaphalocrocis medinalis (Guené e) is a destructive and widespread insect pest throughout the rice growing regions in Asia. The genetics of resistance to RLF in rice is very complex and not thoroughly explored. The present study was conducted to detect the quantitative trait loci (QTL) associated with RLF resistance involving 176 recombinant inbred lines (RILs) of F 8 generation derived from a cross between IR36, a leaffolder susceptible variety and TNAULFR831311, a moderately resistant indica rice culture. Simple sequence repeat (SSR) markers were used to construct specific linkage groups of rice. All the RILs were screened to assess their level of resistance to RLF by measuring the leaf area damaged. Besides this, the length and width of the flag leaf of each RIL were measured since these two parameters were considered as correlated traits to the RLF resistance in rice. All the above parameters observed across the RILs showed quantitative variation.Correlation analysis revealed that damage score based on greenhouse screening was positively correlated with length and width of the flag leaf. Out of 364 SSR markers analysed, 90 were polymorphic between the parents. Multi-point analysis carried out on segregating 69 SSR marker loci linkage group wise resulted in construction of linkage map with eleven groups of 42 SSR markers. Through single marker analysis, 19 SSR markers were found to have putative association with the three phenotypic traits studied. Of these markers, RM472 was identified as a locus having major effect on RLF resistance trait based on length of the flag leaf. Interval mapping detected two QTLs on linkage group 1. Among these QTLs, the QTL flanked by RM576-RM3412 were found to be associated with width of the flag leaf and RLF resistance. The putative SSR markers associated with leaffolder resistance identified in the present study may be one of the loci contributing resistance to RLF in rice.
Honey is a natural product widely used by humans due to its sweet taste and health benefits produced by bees from nectar and honey dew of various plants. To establish and increase the production of honey one must know the plants that take part in the production of honey. In this study pollen analysis of forest honey samples from northern part of Malaysia was carried out to determine the botanical sources playing role in the production of honey in that region. The pollen samples were acetolyzed and identified microscopically. Out of the three samples studied Baling sample was unifloral having Mimosa scabrella as predominant pollen while Jeli and Gerik samples are multifloral containing Tipo myrcia and Elais guineensis as major secondary pollen. Fabaceae family represented four pollen types and accounted 80% of pollen in Baling sample and 34 % in Gerik sample while completely absent in Jeli sample. These results showed the dominance of plants from Fabaceae family in honey production. All the samples analysed have Albizia falcataria, Eupatorium sp., Sparganium typha, Tilia sp. and Tipo myrcia in common indicating that these plants are present in all the three places and these results also can be used as a tool in geographical identification of North Malaysian honey from others.
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